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Literature summary extracted from

  • Pedersen, H.L.; Willassen, N.P.; Leiros, I.
    The first structure of a cold-adapted superoxide dismutase (SOD): biochemical and structural characterization of iron SOD from Aliivibrio salmonicida (2009), Acta Crystallogr. Sect. F, 65, 84-92.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 gene sodB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) Aliivibrio salmonicida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.15.1.1 purified recombinant enzyme by hanging drop vapour diffusion method, 6.5 mg/ml protein in a solution containing 0.1 M Tris-HCl, 1.4 M sodium citrate pH 8.5, 18°C, few days, X-ray diffraction structure determination and analysis at 1.7 A resolution Aliivibrio salmonicida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 2 O2.- + 2 H+ Aliivibrio salmonicida
-
 O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Aliivibrio salmonicida B6ENP9 gene sodB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 recombinant enzyme from Escherichia coli strain BL21(DE3) Aliivibrio salmonicida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 2 O2.- + 2 H+
-
 Aliivibrio salmonicida O2 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 FeSOD
-
 Aliivibrio salmonicida
1.15.1.1 iron superoxide dismutase
-
 Aliivibrio salmonicida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 additional information
-
 an increased net negative charge on the surface of asFeSOD may explain its lower thermostability compared to the enzyme from Escherichia coli, structure-thermostability relationship, overview Aliivibrio salmonicida
1.15.1.1 57
-
 purified recombinant enzyme, loss of over 50% activity Aliivibrio salmonicida