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Literature summary extracted from

  • Lashkov, A.A.; Gabdoulkhakov, A.G.; Shtil, A.A.; Mikhailov, A.M.
    Crystallization and preliminary X-ray c uridine phosphorylase complexed with 5-fluorouracil (2009), Acta Crystallogr. Sect. F, 65, 601-603.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.3 expression in Escherichia coli strain BL21(DE3) Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.3 enzyme in complex with 5-fluorouracil, hanging-drop vapour-diffusion method, 0.5 ml of reservoir solution containing 0.34 ml 0.1 M Tris-maleate/NaOH buffer, pH 5.5, and 0.16 ml 40% w/v PEG 3350 is mixed with 2 ml protein solution containing 11.3 mg/ml enzyme in 10 mM Tris-HCl buffer, pH 7.3, 0.002 ml H2O, 0.0013 ml reservoir solution, 0.002 ml 100 mM 5-fluorouracil, and 0.0003 ml 2-propanol, 21°C, 1-2 weeks, X-ray diffraction structure determination and analysis at 2.2 A resolution, modeling Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.3 additional information Salmonella enterica subsp. enterica serovar Typhimurium the enzyme also activates pyrimidine-containing drugs, including 5-fluorouracil, mechanism of the enzyme-drug interaction, overview ?
-
?
2.4.2.3 uridine + phosphate Salmonella enterica subsp. enterica serovar Typhimurium
-
uracil + alpha-D-ribose 1-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.3 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.3 recombinant enzyme from Escherichia coli strain BL21(DE3) by hydrophobic interaction and anion exchange chromatography to 96% homogeneity Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.3 additional information the enzyme also activates pyrimidine-containing drugs, including 5-fluorouracil, mechanism of the enzyme-drug interaction, overview Salmonella enterica subsp. enterica serovar Typhimurium ?
-
?
2.4.2.3 uridine + phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium uracil + alpha-D-ribose 1-phosphate
-
?
2.4.2.3 uridine + phosphate cleavage of the N-glycosidic bond Salmonella enterica subsp. enterica serovar Typhimurium uracil + alpha-D-ribose 1-phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.3 UPH
-
Salmonella enterica subsp. enterica serovar Typhimurium