EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.3 | expression in Escherichia coli strain BL21(DE3) | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.2.3 | enzyme in complex with 5-fluorouracil, hanging-drop vapour-diffusion method, 0.5 ml of reservoir solution containing 0.34 ml 0.1 M Tris-maleate/NaOH buffer, pH 5.5, and 0.16 ml 40% w/v PEG 3350 is mixed with 2 ml protein solution containing 11.3 mg/ml enzyme in 10 mM Tris-HCl buffer, pH 7.3, 0.002 ml H2O, 0.0013 ml reservoir solution, 0.002 ml 100 mM 5-fluorouracil, and 0.0003 ml 2-propanol, 21°C, 1-2 weeks, X-ray diffraction structure determination and analysis at 2.2 A resolution, modeling | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.3 | additional information | Salmonella enterica subsp. enterica serovar Typhimurium | the enzyme also activates pyrimidine-containing drugs, including 5-fluorouracil, mechanism of the enzyme-drug interaction, overview | ? | - |
? | |
2.4.2.3 | uridine + phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
uracil + alpha-D-ribose 1-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.3 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.3 | recombinant enzyme from Escherichia coli strain BL21(DE3) by hydrophobic interaction and anion exchange chromatography to 96% homogeneity | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.3 | additional information | the enzyme also activates pyrimidine-containing drugs, including 5-fluorouracil, mechanism of the enzyme-drug interaction, overview | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
2.4.2.3 | uridine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | uracil + alpha-D-ribose 1-phosphate | - |
? | |
2.4.2.3 | uridine + phosphate | cleavage of the N-glycosidic bond | Salmonella enterica subsp. enterica serovar Typhimurium | uracil + alpha-D-ribose 1-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.3 | UPH | - |
Salmonella enterica subsp. enterica serovar Typhimurium |