BRENDA - Enzyme Database show

Crystallization and preliminary X-ray analysis of dihydrodipicolinate synthase from Clostridium botulinum in the presence of its substrate pyruvate

Atkinson, S.C.; Dobson, R.C.; Newman, J.M.; Gorman, M.A.; Dogovski, C.; Parker, M.W.; Perugini, M.A.; Acta Crystallogr. Sect. F 65, 253-255 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.3.3.7
inserted into the vector pET11a and transformed into Escherichia coli strain CodonPlus BL21 (DE3)
Clostridium botulinum
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.3.3.7
in the presence of its substrate pyruvate, by the sitting-drop vapour diffusion method, to 1.2 A resolution. Belongs to space group C2, in contrast to the unbound form, which has trigonal symmetry. Unit-cell parameters are a = 143.4, b = 54.8, c = 94.3 A , beta = 126.3. The crystal volume per protein weight is 2.3 A3 Da-1 (based on the presence of two monomers in the asymmetric unit), with an estimated solvent content of 46%
Clostridium botulinum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
31530
-
purified enzyme, mass spectrometry
Clostridium botulinum
4.3.3.7
31650
-
mass spectrometry
Clostridium botulinum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Clostridium botulinum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.3.7
by anion-exchange chromatography, hydrophobic interaction and gel filtration
Clostridium botulinum
Storage Stability
EC Number
Storage Stability
Organism
4.3.3.7
-20°C, 20 mM Tris-HCl buffer, pH 8.0
Clostridium botulinum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
-
701515
Clostridium botulinum
dihydrodipicolinate + 2 H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.3.3.7
tetramer
crystallography
Clostridium botulinum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
inserted into the vector pET11a and transformed into Escherichia coli strain CodonPlus BL21 (DE3)
Clostridium botulinum
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.3.3.7
in the presence of its substrate pyruvate, by the sitting-drop vapour diffusion method, to 1.2 A resolution. Belongs to space group C2, in contrast to the unbound form, which has trigonal symmetry. Unit-cell parameters are a = 143.4, b = 54.8, c = 94.3 A , beta = 126.3. The crystal volume per protein weight is 2.3 A3 Da-1 (based on the presence of two monomers in the asymmetric unit), with an estimated solvent content of 46%
Clostridium botulinum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
31530
-
purified enzyme, mass spectrometry
Clostridium botulinum
4.3.3.7
31650
-
mass spectrometry
Clostridium botulinum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
by anion-exchange chromatography, hydrophobic interaction and gel filtration
Clostridium botulinum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.3.3.7
-20°C, 20 mM Tris-HCl buffer, pH 8.0
Clostridium botulinum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
L-aspartate 4-semialdehyde + pyruvate
-
701515
Clostridium botulinum
dihydrodipicolinate + 2 H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.3.7
tetramer
crystallography
Clostridium botulinum