Literature summary extracted from
Padmanabhan, B.; Strange, R.W.; Antonyuk, S.V.; Ellis, M.J.; Hasnain, S.S.; Iino, H.; Agari, Y.; Bessho, Y.; Yokoyama, S.
Structure of dihydrodipicolinate synthase from Methanocaldococcus jannaschii (2009), Acta Crystallogr. Sect. F, 65, 1222-1226.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.3.3.7 |
into the pET-21a expression vector and introduced into Escherichia coli Rosetta (DE3) strain |
Methanocaldococcus jannaschii |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.3.3.7 |
by the oil-batch method at 291 K, to 2.2 A resolution. Belongs to space group P21 with unit-cell parameters a = 80.5 A, b = 76.5 A, c = 101.9 A, gamma = 106.9 A. The asymmetric unit contains four DHDPS molecules, forming a homotetramer with approximate 222 symmetry. The overall tertiary structure of DHDPS possesses a (beta/alpha)8-barrel fold (TIM barrel) with three additional alpha-helices (alpha9-alpha11) at the C-terminus of the chain. The beta-strands of the barrel form an intrinsic network of hydrogen-bonding interactions with the neighbouring beta-strands and are oriented in the same directions. The functional residue Lys161, which participates in Schiff-base formation, is located within the beta-barrel and the side chain of Tyr132 sits over this residue |
Methanocaldococcus jannaschii |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.3.3.7 |
Methanocaldococcus jannaschii |
Q57695 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.3.3.7 |
by gel filtration and ultrafiltration |
Methanocaldococcus jannaschii |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.3.3.7 |
homotetramer |
crystallography |
Methanocaldococcus jannaschii |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.3.3.7 |
DHDPS |
- |
Methanocaldococcus jannaschii |
4.3.3.7 |
dihydrodipicolinate synthase |
- |
Methanocaldococcus jannaschii |