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Literature summary extracted from

  • Strange, R.W.; Antonyuk, S.V.; Ellis, M.J.; Bessho, Y.; Kuramitsu, S.; Yokoyama, S.; Hasnain, S.S.
    The structure of an archaeal ribose-5-phosphate isomerase from Methanocaldococcus jannaschii (MJ1603) (2009), Acta Crystallogr. Sect. F, 65, 1214-1217.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.6 expression of MJ1603 in Escherichia coli strain Rosetta (DE3) Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.6 purified recombinant MJ1603, microbatch-under-oil method, 0.0005 ml of 8.1 mg/ml protein in 20 mM Tris-HCl buffer, pH 8.0, containing 200 mM NaCl are mixed with 0.0005 ml of crystallization reagent, consisting of 0.1 M acetate, pH 4.5 containing 40% v/v 1,2-propanediol and 0.05 M calcium acetate, 18°C. The mixture is covered with 0.015 ml silicone and paraffin oil, X-ray diffraction structure determination and analysis at 1.78 A resolution, molecular replacement and modeling Methanocaldococcus jannaschii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.6 D-ribose 5-phosphate Methanocaldococcus jannaschii
-
 D-ribulose 5-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.6 Methanocaldococcus jannaschii Q58998 strain DSM 2661
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.6 recombinant MJ1603 from Escherichia coli strain Rosetta (DE3) by anion exchange and hydroxyapatite chromatography, and gel filtration Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.6 D-ribose 5-phosphate
-
 Methanocaldococcus jannaschii D-ribulose 5-phosphate
-
 r
5.3.1.6 D-ribose 5-phosphate the open configuration of ribose 5-phosphate is required for isomerization and is reversibly converted to ribulose 5-phosphate Methanocaldococcus jannaschii D-ribulose 5-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.6 tetramer RpiA crystal structure, overview Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
5.3.1.6 MJ1603
-
 Methanocaldococcus jannaschii
5.3.1.6 RpiA
-
 Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
5.3.1.6 metabolism two evolutionary distinct forms of the enzyme, RpiA and RpiB, with different amino acid sequences and molecular weights exist, that both catalyze the reversible conversion of ribose 5-phosphate to ribulose 5-phosphate. RpiA is found in the bacterial, plant and animal kingdoms, whereas RpiB is less widespread and is found in bacterial sources Methanocaldococcus jannaschii