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Literature summary extracted from

  • Jung, J.; Yeom, S.J.; Kim, J.; Kim, J.K.; Natarajan, S.; Ahn, Y.J.; Lim, S.B.; Oh, D.K.; Kang, L.W.
    Overexpression, crystallization and preliminary X-ray crystallographic analysis of D-ribose-5-phosphate isomerase from Clostridium thermocellum (2009), Acta Crystallogr. Sect. F, 65, 1141-1144.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.6 gene rpiB, expression in Escherichia coli strain ER2566 Acetivibrio thermocellus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.6 purified recombinant RpiB, sitting drop vapour diffusion method, 0.001 ml of 7 mg/ml protein in mM Tris-HCl buffer pH 7.5 is mixed with 0.001 ml of reservoir solution, containing 0.05 M Tris, pH 7.0, 10% PEG 8000, 0.15 M MgCl2 and 0.2 M KCl, and equilibrated against 1 ml of reservoir solution, 3 weeks, flash-cooling in liquid nitrogen with a cryoprotectant solution containing 65 mM Tris pH 7.0, 13% PEG 8000, 195 mM MgCl2, 260 mM KCl and 20% glycerol, X-ray diffraction structure determination and analysis at 1.9 A resolution, modeling Acetivibrio thermocellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.6 35000
-
 about, native PAGE, recombinant enzyme Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.6 D-allose Acetivibrio thermocellus
-
 D-psicose
-
 r
5.3.1.6 D-ribose Acetivibrio thermocellus
-
 D-ribulose
-
 r
5.3.1.6 D-ribose 5-phosphate Acetivibrio thermocellus preferred substrate D-ribulose 5-phosphate
-
 r
5.3.1.6 D-talose Acetivibrio thermocellus
-
 D-tagatose
-
 r
5.3.1.6 L-allose Acetivibrio thermocellus
-
 L-psicose
-
 r
5.3.1.6 L-ribose Acetivibrio thermocellus
-
 L-ribulose
-
 r
5.3.1.6 L-talose Acetivibrio thermocellus preferred substrate L-tagatose
-
 r
5.3.1.6 additional information Acetivibrio thermocellus CTRPI has a narrow substrate specificity for rare sugars and reversibly converts aldose substrates containing hydroxyl groups oriented in the same direction to one of the corresponding ketoses. CTRPI prefers aldose substrates such as L-talose, D-ribose and D-allose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.6 Acetivibrio thermocellus
-
 RpiB; gene rpiB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.6 recombinant RpiB from Escherichia coli strain ER2566 by His affinity chromatography Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.6 D-allose
-
 Acetivibrio thermocellus D-psicose
-
 r
5.3.1.6 D-ribose
-
 Acetivibrio thermocellus D-ribulose
-
 r
5.3.1.6 D-ribose 5-phosphate preferred substrate Acetivibrio thermocellus D-ribulose 5-phosphate
-
 r
5.3.1.6 D-talose
-
 Acetivibrio thermocellus D-tagatose
-
 r
5.3.1.6 L-allose
-
 Acetivibrio thermocellus L-psicose
-
 r
5.3.1.6 L-ribose
-
 Acetivibrio thermocellus L-ribulose
-
 r
5.3.1.6 L-talose preferred substrate Acetivibrio thermocellus L-tagatose
-
 r
5.3.1.6 additional information CTRPI has a narrow substrate specificity for rare sugars and reversibly converts aldose substrates containing hydroxyl groups oriented in the same direction to one of the corresponding ketoses. CTRPI prefers aldose substrates such as L-talose, D-ribose and D-allose Acetivibrio thermocellus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.6 dimer 2 x 17500, about, recombinant enzyme, RpiB has an active-site interface between the dimer structures Acetivibrio thermocellus

Synonyms

EC Number Synonyms Comment Organism
5.3.1.6 CTRPI
-
 Acetivibrio thermocellus
5.3.1.6 D-ribose-5-phosphate isomerase
-
 Acetivibrio thermocellus
5.3.1.6 RpiB
-
 Acetivibrio thermocellus

General Information

EC Number General Information Comment Organism
5.3.1.6 metabolism Rpi is involved in D-allose metabolism by converting D-ribose-5-phosphate to D-ribulose-5-phosphate and vice versa in a branch of the pentose phosphate pathway Acetivibrio thermocellus