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Literature summary extracted from

  • Tremblay, L.W.; Blanchard, J.S.
    The 1.9 A structure of the branched-chain amino-acid transaminase (IlvE) from Mycobacterium tuberculosis (2009), Acta Crystallogr. Sect. F, 65, 1071-1077.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.42 expressed in Escherichia coli Rosetta 2 cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.42 hanging drop vapor diffusion method, using 1% (w/v) tryptone, 50 mM HEPES pH 7.0, and 12% (w/v) polyethylene glycol 3350 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.42 39700
-
 2 * 39700, calculated from amino acid sequence Mycobacterium tuberculosis
2.6.1.42 40000
-
 2 * 40000, the biologically active form of IlvE is a homodimer, SDS-PAGE Mycobacterium tuberculosis
2.6.1.42 80000
-
 gel filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Mycobacterium tuberculosis P9WQ75
-
-
2.6.1.42 Mycobacterium tuberculosis H37Rv P9WQ75
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.42 Ni2+ affinity column chromatography and S75 gel filtration Mycobacterium tuberculosis

Subunits

EC Number Subunits Comment Organism
2.6.1.42 homodimer 2 * 39700, calculated from amino acid sequence Mycobacterium tuberculosis
2.6.1.42 homodimer 2 * 40000, the biologically active form of IlvE is a homodimer, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 BcaT
-
 Mycobacterium tuberculosis
2.6.1.42 branched-chain amino-acid transaminase
-
 Mycobacterium tuberculosis
2.6.1.42 IlvE
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.42 pyridoxal 5'-phosphate
-
 Mycobacterium tuberculosis