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Literature summary extracted from

  • Okvist, M.; Sasso, S.; Roderer, K.; Kast, P.; Krengel, U.
    A novel noncovalent complex of chorismate mutase and DAHP synthase from Mycobacterium tuberculosis: protein purification, crystallization and X-ray diffraction analysis (2009), Acta Crystallogr. Sect. F, 65, 1048-1052.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.99.5 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase
-
 Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.54 expression of enzyme with affinity purification tagwith plasmid pKTDS-HN in Escherichia coli KA13 Mycobacterium tuberculosis
5.4.99.5 expressed in Escherichia coli strain KA13 Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.54 in complex with chorismate mutase, hanging-drop method, in 20 mM BTP, pH 7.5, 150 mM NaCl, 0.5 mM tris(2-carboxyethyl)phosphine hydrochloride, 0.2 mM phosphoenolpyruvate and 0.1 mM MnCl2, crystallization after 2 months with no ammonium sulfate and 0.1 M Tris-HCl, pH 7.9-8.0 and PEG 400 or glycerol Mycobacterium tuberculosis
5.4.99.5 alone and in complex with 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, hanging drop vapor diffusion method, using 25% (w/v) PEG 1500 and 0.1 M MMT buffer (L-malic acid, MES, Tris) pH 8.0-9.0 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 51830
-
 expected mass, calculated from the 472 amino acids Mycobacterium tuberculosis
2.5.1.54 51830
-
 observed by ESI-MS Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Mycobacterium tuberculosis
-
 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Mycobacterium tuberculosis O53512
-
-
5.4.99.5 Mycobacterium tuberculosis P9WIC1
-
-
5.4.99.5 Mycobacterium tuberculosis H37Rv P9WIC1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 cells centrifuged and resuspended in 20 mM BTP (1,3-bis[tris(hydroxymethyl)methylamino]propane), pH 7.5, 150 mM NaCl, 0.5 mM TCEP (tris(2-carboxyethyl)phosphine hydrochloride), 0.2 mM phosphoenolpyruvate and 0.1 mM MnCl2, cell lysis with lysozyme and sonication, centrifugation, crude extract subjected to Ni-NTA agarose chromatography, elution with 250 mM imidazole in buffer, further purification by preparative FPLC HiLoad 26/60 Superdex 75 gel-filtration column Mycobacterium tuberculosis
5.4.99.5 ammonium sulfate precipitation, Ni-NTA agarose column chromatography, and Superdex 75 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Mycobacterium tuberculosis 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
 ?
5.4.99.5 Chorismate the enzyme catalyzes the Claisen rearrangement of chorismate to prephenate Mycobacterium tuberculosis Prephenate
-
 ?
5.4.99.5 Chorismate the enzyme catalyzes the Claisen rearrangement of chorismate to prephenate Mycobacterium tuberculosis H37Rv Prephenate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.54 3-deoxy-7-phosphoheptulonate synthase
-
 Mycobacterium tuberculosis
2.5.1.54 DAHP synthase
-
 Mycobacterium tuberculosis
2.5.1.54 MtDS
-
 Mycobacterium tuberculosis
5.4.99.5 Rv0948c
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.5.1.54 metabolism initial step in the shikimate biosynthesis pathway, enhances the catalytic efficiency of chorismate mutase in a noncovalent enzyme complex Mycobacterium tuberculosis