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Literature summary extracted from

  • Usha, V.; Dover, L.G.; Roper, D.I.; Fuetterer, K.; Besra, G.S.
    Structure of the diaminopimelate epimerase DapF from Mycobacterium tuberculosis (2009), Acta Crystallogr. Sect. D, 65, 383-387.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.7 recombinant protein is expressed as His-tegged enzyme in BL21(DE3) Escherichia coli cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.1.7 crystal structure of the ligand-free form refines to a resolution of 2.6 A, 2.5 mM dithiothreitol is present in the crystal drop Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.7 LL-2,6-diaminoheptanedioate Mycobacterium tuberculosis stereo-inversion meso-diaminopimelate the meso-isomer of diaminopimelic acid, a precursor of L-lysine, is a key component of the pentapeptide linker in bacterial peptidoglycan ?
5.1.1.7 LL-2,6-diaminoheptanedioate Mycobacterium tuberculosis H37Rv stereo-inversion meso-diaminopimelate the meso-isomer of diaminopimelic acid, a precursor of L-lysine, is a key component of the pentapeptide linker in bacterial peptidoglycan ?

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.7 Mycobacterium tuberculosis P9WP19
-
-
5.1.1.7 Mycobacterium tuberculosis H37Rv P9WP19
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.7 by using nickel-chelate affinity chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.7 LL-2,6-diaminoheptanedioate stereo-inversion Mycobacterium tuberculosis meso-diaminopimelate the meso-isomer of diaminopimelic acid, a precursor of L-lysine, is a key component of the pentapeptide linker in bacterial peptidoglycan ?
5.1.1.7 LL-2,6-diaminoheptanedioate stereo-inversion Mycobacterium tuberculosis H37Rv meso-diaminopimelate the meso-isomer of diaminopimelic acid, a precursor of L-lysine, is a key component of the pentapeptide linker in bacterial peptidoglycan ?

Synonyms

EC Number Synonyms Comment Organism
5.1.1.7 DAP epimerase
-
 Mycobacterium tuberculosis
5.1.1.7 DapF
-
 Mycobacterium tuberculosis
5.1.1.7 diaminopimelate epimerase
-
 Mycobacterium tuberculosis
5.1.1.7 MtDapF
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
5.1.1.7 physiological function synthesis of L-lysine Mycobacterium tuberculosis