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Literature summary extracted from

  • Wei, J.F.; Yang, H.W.; Wei, X.L.; Qiao, L.Y.; Wang, W.Y.; He, S.H.
    Purification, characterization and biological activities of the L-amino acid oxidase from Bungarus fasciatus snake venom (2009), Toxicon, 54, 262-271.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.2 0.04
-
 L-Asp pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 0.05
-
 L-Tyr pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 0.13
-
 L-Phe pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 0.27
-
 L-Trp pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 1.44
-
 L-Glu pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 1.64
-
 L-Ile pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 4.84
-
 L-His pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 5.88
-
 L-Gln pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 15
-
 L-Met pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 18.75
-
 L-Arg pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 19.07
-
 L-Asn pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 21.5
-
 L-Lys pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 28.3
-
 L-Ala pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 60.7
-
 L-Leu pH 8.5, 25°C Bungarus fasciatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.3.2 extracellular venom Bungarus fasciatus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 55000
-
 1 * 55000, SDS-PAGE Bungarus fasciatus
1.4.3.2 70000
-
 gel filtration Bungarus fasciatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.2 additional information Bungarus fasciatus LAAO exhibits a cytotoxic effect on A549 cells and causes up to 41.2% apoptosis of A549 cells, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Bungarus fasciatus
-
 llected from Guangxi Province, P.R. China
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2 native enzyme from venom by anion exchange and heparin affinity chromatography Bungarus fasciatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.2 venom
-
 Bungarus fasciatus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-Ala + H2O + O2
-
 Bungarus fasciatus 2-oxopropanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Arg + H2O + O2
-
 Bungarus fasciatus 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Asn + H2O + O2
-
 Bungarus fasciatus 2-oxosuccinamic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Asp + H2O + O2
-
 Bungarus fasciatus oxaloacetate + NH3 + H2O2
-
 ?
1.4.3.2 L-Gln + H2O + O2
-
 Bungarus fasciatus 5-amino-2,5-dioxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Glu + H2O + O2
-
 Bungarus fasciatus 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.2 L-His + H2O + O2
-
 Bungarus fasciatus 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Ile + H2O + O2
-
 Bungarus fasciatus 3-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Leu + H2O + O2
-
 Bungarus fasciatus 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Lys + H2O + O2
-
 Bungarus fasciatus 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Met + H2O + O2
-
 Bungarus fasciatus 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Phe + H2O + O2
-
 Bungarus fasciatus phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-Trp + H2O + O2
-
 Bungarus fasciatus indol-3-pyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-Tyr + H2O + O2
-
 Bungarus fasciatus 4-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 additional information LAAO exhibits a cytotoxic effect on A549 cells and causes up to 41.2% apoptosis of A549 cells, overview Bungarus fasciatus ?
-
 ?
1.4.3.2 additional information LAAO is very active against L-Tyr, L-Asp, L-Phe, L-Glu, L-Trp, L-His, L-Gln, L-Ile, L-Met, L-Leu and moderately active against L-Lys, L-Arg, L-Ala and L-Asn, overview Bungarus fasciatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 monomer 1 * 55000, SDS-PAGE Bungarus fasciatus

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 LAAO
-
 Bungarus fasciatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.2 25
-
 assay at Bungarus fasciatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.2 47.49
-
 L-Asp pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 66.7
-
 L-Ile pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 84.08
-
 L-Asn pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 98.82
-
 L-Trp pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 100.6
-
 L-Tyr pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 142.6
-
 L-Phe pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 228.8
-
 L-Arg pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 293
-
 L-Ala pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 318.9
-
 L-Lys pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 430.4
-
 L-Gln pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 483.1
-
 L-His pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 589.3
-
 L-Met pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 790.5
-
 L-Glu pH 8.5, 25°C Bungarus fasciatus
1.4.3.2 1025
-
 L-Leu pH 8.5, 25°C Bungarus fasciatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.2 8.5
-
 assay at Bungarus fasciatus