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Literature summary extracted from

  • Wuosmaa, A.M.; Hager, L.P.
    Methyl chloride transferase: a carbocation route for biosynthesis of halometabolites (1990), Science, 249, 160-162.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.165 0.016
-
S-adenosyl-L-methionine cosubstrate: bromide Endocladia muricata
2.1.1.165 5
-
bromide
-
Endocladia muricata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.165 20000 25000 gel filtration Endocladia muricata

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.165 Endocladia muricata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.165 the purification is achieved by an 80 to 100% ammonium sulfate precipitation step followed by a high-performance liquid chromatography gel filtration step on a 60 cm by 2.15 cm preparative Bio-Sil SEC-250 column. The column is eluted with 10 mM phosphate buffer, pH 7.0 Endocladia muricata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.165 0.000555
-
partially purified enzyme, synthesis of methyl bromide Endocladia muricata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.165 additional information fluoride is not a substrate Endocladia muricata ?
-
?
2.1.1.165 S-adenosyl-L-methionine + bromide the rate of production of methyl bromide is 135fold lower than production of methyl iodide Endocladia muricata S-adenosyl-L-homocysteine + methyl bromide
-
?
2.1.1.165 S-adenosyl-L-methionine + chloride the rate of production of methyl chloride is 270fold lower than production of methyl iodide Endocladia muricata S-adenosyl-L-homocysteine + methyl chloride
-
?
2.1.1.165 S-adenosyl-L-methionine + iodide iodide is the preferred substrate Endocladia muricata S-adenosyl-L-homocysteine + methyl iodide
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.165 methyl chloride transferase
-
Endocladia muricata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.165 7.5 7.6 synthesis of methyl bromide Endocladia muricata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.165 6 9 pH 6.0: about 40% of maximal activity, pH 9.0: about 35% of maximal activity, at pH greater than 9.2 no methyl bromide synthesis detected Endocladia muricata