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Literature summary extracted from

  • Galkin, A.; Kulakova, L.; Wu, R.; Gong, M.; Dunaway-Mariano, D.; Herzberg, O.
    X-ray structure and kinetic properties of ornithine transcarbamoylase from the human parasite Giardia lamblia (2009), Proteins Struct. Funct. Bioinform., 76, 1049-1053.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.3 expression in Escherichia coli Giardia intestinalis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.3.3 one protein molecule per asymmetric unit, trimeric structure Giardia intestinalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.3 delta-N-(phosphonacetyl)-L-ornithine
-
 Giardia intestinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.3 0.11
-
 L-ornithine pH 8.0, 37°C Giardia intestinalis
2.1.3.3 0.13
-
 Carbamoyl phosphate pH 8.0, 37°C Giardia intestinalis
2.1.3.3 2.3
-
 phosphate pH 8.0, 37°C Giardia intestinalis
2.1.3.3 10.4
-
 L-citrulline pH 8.0, 37°C Giardia intestinalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 36494
-
 3 * 36494, calculated Giardia intestinalis
2.1.3.3 90000
-
 gel filtration Giardia intestinalis

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Giardia intestinalis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Giardia intestinalis phosphate + L-citrulline
-
 r
2.1.3.3 phosphate + L-citrulline
-
 Giardia intestinalis carbamoyl phosphate + L-ornithine
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.3.3 trimer 3 * 36494, calculated Giardia intestinalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.3.3 7.9
-
 phosphate pH 8.0, 37°C Giardia intestinalis
2.1.3.3 8.1
-
 L-citrulline pH 8.0, 37°C Giardia intestinalis
2.1.3.3 82
-
 Carbamoyl phosphate pH 8.0, 37°C Giardia intestinalis
2.1.3.3 94.2
-
 L-ornithine pH 8.0, 37°C Giardia intestinalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.3.3 0.000025
-
 delta-N-(phosphonacetyl)-L-ornithine pH 8.0, 37°C Giardia intestinalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.3.3 780
-
 L-citrulline pH 8.0, 37°C Giardia intestinalis
2.1.3.3 3400
-
 phosphate pH 8.0, 37°C Giardia intestinalis
2.1.3.3 650000
-
 Carbamoyl phosphate pH 8.0, 37°C Giardia intestinalis
2.1.3.3 880000
-
 L-ornithine pH 8.0, 37°C Giardia intestinalis