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Literature summary extracted from
- Heterologous expression in Escherichia coli of Neurospora crassa neutral trehalase as an active enzyme (2009), Protein Expr. Purif., 65, 185-189.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.28 | expression in Escherichia coli | Neurospora crassa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.28 | Ag+ | - |
Neurospora crassa |  |
| 3.2.1.28 | Al3+ | - |
Neurospora crassa | |
| 3.2.1.28 | ATP | - |
Neurospora crassa | |
| 3.2.1.28 | Co2+ | - |
Neurospora crassa | |
| 3.2.1.28 | Cu2+ | - |
Neurospora crassa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.28 | 42 | - |
alpha,alpha-trehalose | pH 7.0, 30°C | Neurospora crassa | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.28 | Ca2+ | absolutely required | Neurospora crassa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 81000 | - |
gel filtration | Neurospora crassa |
| 3.2.1.28 | 84000 | - |
1 * 84000, SDS-PAGE and calculated | Neurospora crassa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.28 | Neurospora crassa | O42783 | neutral trehalase | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.28 | phosphoprotein | enzyme is phosphorylated by cAMP-dependent protein kinase, but not significantly activated | Neurospora crassa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 80 | 150 | pH 7.0, 30°C | Neurospora crassa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.28 | alpha,alpha-trehalose + H2O | - |
Neurospora crassa | 2 D-glucopyranose | - |
? | |
| 3.2.1.28 | additional information | enzyme is absolutely specific for trehalose | Neurospora crassa | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.28 | monomer | 1 * 84000, SDS-PAGE and calculated | Neurospora crassa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 30 | - |
- |
Neurospora crassa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 40 | - |
half-life 2.5 min | Neurospora crassa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 7 | - |
- |
Neurospora crassa |
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Release 2023.1 (January 2023)
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