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Literature summary extracted from

  • Shahravan, S.H.; Qu, X.; Chan, I.S.; Shin, J.A.
    Enhancing the specificity of the enterokinase cleavage reaction to promote efficient cleavage of a fusion tag (2008), Protein Expr. Purif., 59, 314-319.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.9 additional information changing pH, temperature, or the amount of the enzyme has no effect on cleavage pattern synthetic construct
3.4.21.9 Urea addition of urea (1-4 M) greatly improves enterokinase cleavage specificity at the canonical site and reduces adventitious cleavage synthetic construct

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.9 Urea use of higher amounts of urea (ca. 5 M) has an inhibitory effect on the enzyme activity synthetic construct

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.9 synthetic construct
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.9 AhR6-C/EBP hybrid of the AhR basic region and C/EBP leucine zipper. Reaction under standard conditions (at 37°C in 50 mM Tris buffer, pH 7.6) is rapid and somewhat nonspecific. After just 15 min incubation, 82% of the protein has been cleaved at multiple sites, prolonged incubation causes further degradation of cleavage products synthetic construct ?
-
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Synonyms

EC Number Synonyms Comment Organism
3.4.21.9 enterokinase
-
synthetic construct