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Literature summary extracted from
- Cellobiose dehydrogenase production by the mycelial culture of the mushroom Termitomyces clypeatus (2008), Process Biochem., 43, 634-641.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.18 | 0.01861 | - |
cytochrome c | - |
Termitomyces clypeatus |  |
| 1.1.99.18 | 0.02253 | - |
2,6-dichlorophenol indophenol | - |
Termitomyces clypeatus | |
| 1.1.99.18 | 0.1379 | - |
ferricyanide | - |
Termitomyces clypeatus | |
| 1.1.99.18 | 0.181 | - |
cellobiose | - |
Termitomyces clypeatus | |
| 1.1.99.18 | 0.1905 | - |
cellotriose | - |
Termitomyces clypeatus | |
| 1.1.99.18 | 0.2105 | - |
cellotetraose | - |
Termitomyces clypeatus | |
| 1.1.99.18 | 0.2222 | - |
cellopentaose | - |
Termitomyces clypeatus | |
| 1.1.99.18 | 0.3846 | - |
alpha-lactose | - |
Termitomyces clypeatus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 100000 | - |
gel filtration | Termitomyces clypeatus |
| 1.1.99.18 | 101000 | - |
SDS-PAGE | Termitomyces clypeatus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.18 | Termitomyces clypeatus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.18 | ultrafiltration, gel filtration, hydrophobic interaction chromatography, gel filtration | Termitomyces clypeatus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 16.76 | - |
- |
Termitomyces clypeatus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.18 | alpha-lactose + 2,6-dichlorophenol indophenol | - |
Termitomyces clypeatus | ? | - |
? | |
| 1.1.99.18 | cellobiose + 2,6-dichlorophenol indophenol | - |
Termitomyces clypeatus | ? | - |
? | |
| 1.1.99.18 | cellobiose + cytochrome c | - |
Termitomyces clypeatus | ? | - |
? | |
| 1.1.99.18 | cellobiose + ferricyanide | - |
Termitomyces clypeatus | ? | - |
? | |
| 1.1.99.18 | cellopentaose + 2,6-dichlorophenol indophenol | - |
Termitomyces clypeatus | ? | - |
? | |
| 1.1.99.18 | cellotetraose + 2,6-dichlorophenol indophenol | - |
Termitomyces clypeatus | ? | - |
? | |
| 1.1.99.18 | cellotriose + 2,6-dichlorophenol indophenol | - |
Termitomyces clypeatus | ? | - |
? | |
| 1.1.99.18 | lactose + H2O + acceptor | - |
Termitomyces clypeatus | lactobionic acid + reduced acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | monomer | 1 * 100000, gel filtration, 1 * 101000, SDS-PAGE | Termitomyces clypeatus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | cellobiose dehydrogenase | - |
Termitomyces clypeatus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 30 | - |
pH 5.0 | Termitomyces clypeatus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 50 | - |
50% of activity is lost when incubated at 50°C for 30 min at pH 5.0 | Termitomyces clypeatus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.18 | 8.88 | - |
cellotriose | - |
Termitomyces clypeatus | |
| 1.1.99.18 | 8.88 | - |
cellotetraose | - |
Termitomyces clypeatus | |
| 1.1.99.18 | 9.31 | - |
cellopentaose | - |
Termitomyces clypeatus | |
| 1.1.99.18 | 14.22 | - |
alpha-lactose | - |
Termitomyces clypeatus | |
| 1.1.99.18 | 16.01 | - |
2,6-dichlorophenol indophenol | - |
Termitomyces clypeatus | |
| 1.1.99.18 | 17.65 | - |
cellobiose | - |
Termitomyces clypeatus | |
| 1.1.99.18 | 19.69 | - |
cytochrome c | - |
Termitomyces clypeatus | |
| 1.1.99.18 | 31.36 | - |
ferricyanide | - |
Termitomyces clypeatus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 4.5 | 5 | depending on the electron acceptor | Termitomyces clypeatus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 2 | 8 | at least for 12 h, assayed with 2,6-dichlorophenol indophenol | Termitomyces clypeatus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.18 | FAD | - |
Termitomyces clypeatus | |
| 1.1.99.18 | heme | - |
Termitomyces clypeatus | |
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