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Literature summary extracted from

  • Marty, L.; Siala, W.; Schwarzlaender, M.; Fricker, M.D.; Wirtz, M.; Sweetlove, L.J.; Meyer, Y.; Meyer, A.J.; Reichheld, J.P.; Hell, R.
    The NADPH-dependent thioredoxin system constitutes a functional backup for cytosolic glutathione reductase in Arabidopsis (2009), Proc. Natl. Acad. Sci. USA, 106, 9109-9114.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.7 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 1,3-Bis(2-chloroethyl)-1-nitrosourea significant inhibition of recombinant isoform GR1 is observed at high concentrations of 1 mM and above Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.033
-
 NADPH in 100 mM K2HPO4/KH2PO4 buffer, at pH 7.4 Arabidopsis thaliana
1.8.1.7 0.077
-
 glutathione disulfide in 100 mM K2HPO4/KH2PO4 buffer, at pH 7.4 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.7 chloroplast isoform GR2 Arabidopsis thaliana 9507
-
1.8.1.7 cytosol isoform GR1 Arabidopsis thaliana 5829
-
1.8.1.7 mitochondrion isoform GR2 Arabidopsis thaliana 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 57100
-
 2 * 57100, calculated for GR1 including the N-terminal His-tag Arabidopsis thaliana
1.8.1.7 60000
-
 2 * 60000, SDS-PAGE Arabidopsis thaliana
1.8.1.7 120000
-
 purified native isoform GR1, gel filtration Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7 Ni2+-NTA column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH + H+
-
 Arabidopsis thaliana glutathione + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.7 homodimer 2 * 60000, SDS-PAGE Arabidopsis thaliana
1.8.1.7 homodimer 2 * 57100, calculated for GR1 including the N-terminal His-tag Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.8.1.7 glutathione reductase
-
 Arabidopsis thaliana
1.8.1.7 GR1 cytosolic isoform, about 65% of the total glutathione reductase activity is attributed to isoform GR1 Arabidopsis thaliana
1.8.1.7 GR2 isoform dual-targeted to chloroplasts and mitochondria, about 35% of the total glutathione reductase activity is attributed to isoform GR2 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 7.2 7.6
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 NADPH
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.8.1.7 malfunction deletion of isoform GR1 prevents survival due to a pollen lethal phenotype Arabidopsis thaliana