Literature summary extracted from
Gallazzini, M.; Ferraris, J.D.; Burg, M.B.
GDPD5 is a glycerophosphocholine phosphodiesterase that osmotically regulates the osmoprotective organic osmolyte GPC (2008), Proc. Natl. Acad. Sci. USA, 105, 11026-11031.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.4.44 |
expression in mIMCD-3 cell: GDPD5 expression reduces (mRNA level) when osmolarity is increased from 300 to 700 mosmol/kg by adding NaCl (200 mosmol/kg) and urea (200 mosmol/kg) in combination. HEK293 cells transfected with GDPD5: recombinant GDPD5 is perinuclear. GDPD5 colocalizes with neuropathy target esterase in the ER of HEK293 cells. Expression of GDPD5 increases GPC-PDE activity (specific siRNA assay to knock down GDPD5, mIMCD-3 cells) |
Mus musculus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.4.44 |
Urea |
in vivo: activity decreases in vivo (mIMCD-3 cell), measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine. in vitro: no directl inhibition of GDPD5 activity, GPC-PDE in vitro activity is reduced by over 60% in immuno-precipitates from HEK293 cells exposed to high urea concentration. |
Mus musculus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.4.44 |
KCl |
in vitro: no direct decrease in GDPD5 activity, measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine |
Mus musculus |
|
3.1.4.44 |
NaCl |
in vivo: activity decreases (mIMCD-3 cell), measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine. in vitro: no direct decrease in GDPD5 activity, GPC-PDE in vitro activity is reduced by over 60% in immuno-precipitates from HEK293 cells exposed to high NaCl concentration. |
Mus musculus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.4.44 |
Mus musculus |
Q640M6 |
- |
- |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.1.4.44 |
IMCD-3 cell |
- |
Mus musculus |
- |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.4.44 |
glycerophosphodiester phosphodiesterase domain containing 5 |
GDPD5, BC024955 |
Mus musculus |