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Literature summary extracted from

  • Egea, P.F.; Tsuruta, H.; de Leon, G.P.; Napetschnig, J.; Walter, P.; Stroud, R.M.
    Structures of the signal recognition particle receptor from the archaeon Pyrococcus furiosus: implications for the targeting step at the membrane (2008), PLoS ONE, 3, e3619.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.5.4 expressed in Escherichia coli BL21(DE3)-Rosetta2 cells Pyrococcus furiosus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.5.4 free and GDP-magnesium-bound forms, hanging drop vapour diffusion method, the hexagonal form grows in 1.1-1.5 M ammonium phosphate and 100 mM sodium acetate pH 5.0. The monoclinic form grows in 0.9-1.2 M lithium sulfate, 0.4-0.6 M ammonium sulfate, and 100 mM sodium citrate pH 5.0. For the GDP-bound structure, best crystals grow in 14-17% (w/v) PEG 8000 and 100 mM Tris pH 8.0 Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.5.4 34900
-
 apo-enzyme, analytical ultracentrifugation and small-angle X-ray scattering Pyrococcus furiosus
3.6.5.4 35810
-
 apo-enzyme, calculated from amino acid sequence Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.4 Pyrococcus furiosus Q8U051
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.5.4 heat selective precipitation, cobalt-chelating affinity chromatography, gel filtration, and ion-exchange chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.4 GTP + H2O
-
 Pyrococcus furiosus GDP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.5.4 FtsY
-
 Pyrococcus furiosus
3.6.5.4 signal recognition particle receptor
-
 Pyrococcus furiosus
3.6.5.4 SRP
-
 Pyrococcus furiosus