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Literature summary extracted from

  • Egea, P.F.; Napetschnig, J.; Walter, P.; Stroud, R.M.
    Structures of SRP54 and SRP19, the two proteins that organize the ribonucleic core of the signal recognition particle from Pyrococcus furiosus (2008), PLoS ONE, 3, e3528.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.5.4 expressed in Escherichia coli B834(DE3)-Rosetta2 cells Pyrococcus furiosus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.5.4 GDP-bound subunit SRP54 and free subunit SRP19 are crystallized by hanging drop vapour diffusion method, crystals of SRP54 grow in 1.0-1.3 M lithium sulfate and 100 mM sodium acetate pH 5.0, crystals of SRP19 grow in 1.2-1.3 M sodium malonate and 100 mM sodium acetate pH 5.0 Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.4 Pyrococcus furiosus Q8U070
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.5.4 heat selective precipitation, cobalt-chelating affinity chromatography, gel filtration, and ion-exchange chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.4 GTP + H2O
-
 Pyrococcus furiosus GDP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.5.4 dimer subunit SRP54, X-ray crystallography Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.6.5.4 Ffh
-
 Pyrococcus furiosus
3.6.5.4 signal recognition particle
-
 Pyrococcus furiosus
3.6.5.4 SRP
-
 Pyrococcus furiosus
3.6.5.4 SRP19 accessory subunit of the signal recognition particle, SRP54 and SRP19 are the two RNA binding components forming the core of the signal recognition particle, SRP19 acts as a molecular scaffold and a chaperone, assisting the SRP RNA in adopting the conformation required for its optimal interaction with the essential subunit SRP54, and proper assembly of a functional SRP Pyrococcus furiosus
3.6.5.4 SRP54 GTPase subunit of the signal recognition particle, SRP54 and SRP19 are the two RNA binding components forming the core of the signal recognition particle Pyrococcus furiosus