Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Altenbach, D.; Rudino-Pinera, E.; Olvera, C.; Boller, T.; Wiemken, A.; Ritsema, T.
    An acceptor-substrate binding site determining glycosyl transfer emerges from mutant analysis of a plant vacuolar invertase and a fructosyltransferase (2009), Plant Mol. Biol., 69, 47-56.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.99 expressed in Pichia pastoris Lolium arundinaceum
3.2.1.26 Pichia pastoris with vector pPICZalphaC Allium cepa

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.99 C203N the mutant has an almost doubled relative hydrolytic activity in comparison to wild type enzyme Lolium arundinaceum
2.4.1.99 L322F the mutant shows wild type activity Lolium arundinaceum
2.4.1.99 M173L the mutant shows wild type activity Lolium arundinaceum
2.4.1.99 W362Y the mutant has an almost doubled relative hydrolytic activity in comparison to wild type enzyme Lolium arundinaceum
2.4.1.99 Y248V the mutant shows no activity Lolium arundinaceum
2.4.1.99 Y317W the mutant shows wild type activity Lolium arundinaceum
2.4.1.99 Y408W the mutant shows low activity Lolium arundinaceum
3.2.1.26 F348L wild-type activity Allium cepa
3.2.1.26 L201M wild-type activity Allium cepa
3.2.1.26 N251C wild-type activity Allium cepa
3.2.1.26 S457N wild-type activity Allium cepa
3.2.1.26 V276Y no activity Allium cepa
3.2.1.26 W343Y wild-type activity Allium cepa
3.2.1.26 W440Y almost doubled transglycosylation activity Allium cepa
3.2.1.26 Y394F no activity Allium cepa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.99 5.8
-
 sucrose wild type enzyme Lolium arundinaceum
3.2.1.26 5.8
-
 sucrose pH 5.6, 28°C, wild-type Allium cepa
3.2.1.26 8.8
-
 sucrose pH 5.6, 28°C, W440Y Allium cepa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.26 vacuole
-
 Allium cepa 5773
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.99 Lolium arundinaceum Q9FSV7 before known as Festuca arundinacea
-
3.2.1.26 Allium cepa O81083
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 centrifugation of cell culture medium, supernatants diluted in 50 mM MES-buffer, pH 5.6 Allium cepa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.99 additional information 1-SST shows relatively low hydrolase activity at all offered sucrose concentrations as compared to the glycosyltransferase activity Lolium arundinaceum ?
-
 ?
2.4.1.99 sucrose + sucrose
-
 Lolium arundinaceum D-glucose + 1-kestose
-
 ?
3.2.1.26 sucrose + H2O 50 mM MES-buffer, pH 5.6, 20 min, 28°C Allium cepa D-fructose + D-glucose at sucrose concentrations higher than 100 mM distinct but small amount of 1-kestose is found as transglycosylation product ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.99 1-SST
-
 Lolium arundinaceum
2.4.1.99 sucrose:sucrose 1-fructosyltransferase
-
 Lolium arundinaceum
3.2.1.26 acINV Allium cepa invertase Allium cepa
3.2.1.26 Vacuolar invertase
-
 Allium cepa