EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.81 | PCR-amplification of cDNA from isolated RNA, PCR-products of enzyme-GFP fusion constructs are cloned into vector pPENSOTG and introduced into Arabidopsis protoplasts by polyethylene glycol-mediated transformation, complementation constructs are produced by joining PCR fragments of the enzyme promoter with the enzyme-GFP cDNA, cloned into vector pBIB-HYG, introduced into Agrobacterium GV3101, transformed into homozygous mutant plants via the floral dipping method | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.81 | additional information | mutant plants are complemented with enzyme construct fused to GFP at C-terminus under native promoter or a constitutive 35S-promoter control, suppressing the mutant phenotype, N-terminal deletion of the first 39 amino acids leads to location in the cytosol instead of chloroplast membrane (crucial localization signal) | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.13.81 | chloroplast membrane | N-terminal 39 amino acids are essential for chloroplast localization of the enzyme | Arabidopsis thaliana | 31969 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.81 | magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | Arabidopsis thaliana | - |
protochlorophyllide a + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.81 | Arabidopsis thaliana | Q9M591 | chl27-t knock-down mutant | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.13.81 | leaf | - |
Arabidopsis thaliana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.81 | magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 | - |
Arabidopsis thaliana | protochlorophyllide a + NADP+ + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.81 | aerobic Mg-protoporphyrin IX monomethyl ester cyclase | - |
Arabidopsis thaliana |
1.14.13.81 | CHL27 | - |
Arabidopsis thaliana |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.13.81 | malfunction | the Arabidopsis chl27-t knock-down mutant has a T-DNA insertion within the promoter region reducing mRNA levels dramatically, growth is slowed down and plants look pale due to chloroplast development defects (unstacking of thylakoid membranes), chlorophyll fluorescence analysis shows a low photosynthetic activity, numerous nuclear genes involved in pigment biosynthesis, photosynthesis (light-harvesting complex I and II, photo system I and II), for iron acquisition, carbon fixation, cold acclimation, and for electron carriers are repressed, stress and detoxifying related gene expression is affected most prominently, more than 600 genes are repressed, a similar number of genes are induced | Arabidopsis thaliana |
1.14.13.81 | metabolism | enzyme is involved in the protochlorophyllide a synthesis | Arabidopsis thaliana |