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Literature summary extracted from

  • Aly, R.; Cholakh, H.; Joel, D.M.; Leibman, D.; Steinitz, B.; Zelcer, A.; Naglis, A.; Yarden, O.; Gal-On, A.
    Gene silencing of mannose 6-phosphate reductase in the parasitic weed Orobanche aegyptiaca through the production of homologous dsRNA sequences in the host plant (2009), Plant Biotechnol. J., 7, 487-498.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.224 transgenic tomato plants bearing a gene construct containing a specific 277-bp fragment from M6PR-mRNA, in an inverted-repeat configuration. Eight independent lines containing the M6PR-silencing inverted-repeat construct pBin-IR-M6PR construct are regenerated Phelipanche aegyptiaca

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.224 siRNA transformed tomato plants containing siRNA from the M6PR of Orobanche aegyptiaca show normal growth and development, accumulation of M6PR siRNA in transgenic tomatoes correlates with decreased levels of M6PR mRNA. Amount of endogenous M6PR mRNA in the tubercles and underground shoots of Orobanche aegyptiaca grown on transgenic host plants is reduced by 60%-80%. Concomitant with M6PR mRNA suppression, there is a significant decrease in mannitol level and a significant increase in the percentage of dead Orobanche aegyptiaca tubercles on the transgenic host plants. Detection of mir390, which is involved with cytoplasmic dsRNA processing, indicates to the existence of gene-silencing mechanisms in Orobanche spp. Gene silencing mechanisms are probably involved with the production of decreased levels of M6PR mRNA in the parasites grown on the transformed tomato lines Phelipanche aegyptiaca

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.224 Phelipanche aegyptiaca
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.224 root tubercle
-
 Phelipanche aegyptiaca
-
1.1.1.224 shoot underground Phelipanche aegyptiaca
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.224 D-mannose 6-phosphate + NADPH + H+
-
 Phelipanche aegyptiaca D-mannitol 1-phosphate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.224 M6PR
-
 Phelipanche aegyptiaca
1.1.1.224 mannose 6-phosphate reductase
-
 Phelipanche aegyptiaca