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Literature summary extracted from

  • Itoh, N.; Tsujita, M.; Ando, T.; Hisatomi, G.; Higashi, T.
    Formation and emission of monohalomethanes from marine algae (1997), Phytochemistry, 45, 67-73.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.165 Iodide above 25 mM Pavlova gyrans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.165 0.024
-
 S-adenosyl-L-methionine pH 7.0, 30°C Pavlova gyrans
2.1.1.165 63
-
 Iodide pH 7.0, 30°C Pavlova gyrans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.165 additional information Papenfussiella kuromo marine microalgae are the main oceanic source of methyl bromide. The monohalomethanes produced by marine microalgae are probably important in the global cycling of gaseous organohalogen species, especially bromine and iodine ?
-
 ?
2.1.1.165 additional information Sargassum horneri marine microalgae are the main oceanic source of methyl bromide. The monohalomethanes produced by marine microalgae are probably important in the global cycling of gaseous organohalogen species, especially bromine and iodine ?
-
 ?
2.1.1.165 additional information Pavlova gyrans marine microalgae are the main oceanic source of methyl bromide. The monohalomethanes produced by marine microalgae are probably important in the global cycling of gaseous organohalogen species, especially bromine and iodine. From the viewpoint of stratospheric ozone depletion, methyl bromide is the most destructive compound because it has a high ozone depletion potential ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.165 Papenfussiella kuromo
-
-
-
2.1.1.165 Pavlova gyrans
-
-
-
2.1.1.165 Sargassum horneri
-
-
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.165 -20°C, enzyme in cell extract is unstable and loses activities almost completely upon storage even if dithioerythritol, EDTA, protease inhibitor or glycerol are added to the extracts Papenfussiella kuromo
2.1.1.165 -20°C, enzyme in cell extract is unstable and loses activities almost completely upon storage even if dithioerythritol, EDTA, protease inhibitor or glycerol are added to the extracts Sargassum horneri
2.1.1.165 4°C, enzyme in cell extract is unstable and loses activities almost completely upon storage even if dithioerythritol, EDTA, protease inhibitor or glycerol are added to the extracts Papenfussiella kuromo
2.1.1.165 4°C, enzyme in cell extract is unstable and loses activities almost completely upon storage even if dithioerythritol, EDTA, protease inhibitor or glycerol are added to the extracts Sargassum horneri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.165 additional information marine microalgae are the main oceanic source of methyl bromide. The monohalomethanes produced by marine microalgae are probably important in the global cycling of gaseous organohalogen species, especially bromine and iodine Papenfussiella kuromo ?
-
 ?
2.1.1.165 additional information marine microalgae are the main oceanic source of methyl bromide. The monohalomethanes produced by marine microalgae are probably important in the global cycling of gaseous organohalogen species, especially bromine and iodine Sargassum horneri ?
-
 ?
2.1.1.165 additional information marine microalgae are the main oceanic source of methyl bromide. The monohalomethanes produced by marine microalgae are probably important in the global cycling of gaseous organohalogen species, especially bromine and iodine. From the viewpoint of stratospheric ozone depletion, methyl bromide is the most destructive compound because it has a high ozone depletion potential Pavlova gyrans ?
-
 ?
2.1.1.165 additional information no activity with chloride, no activity with L-methionine, S-methyl methionine or dimethylsulfoniopropionate Pavlova gyrans ?
-
 ?
2.1.1.165 S-adenosyl-L-methionine + bromide
-
 Pavlova gyrans S-adenosyl-L-homocysteine + methyl bromide
-
 ?
2.1.1.165 S-adenosyl-L-methionine + iodide
-
 Pavlova gyrans S-adenosyl-L-homocysteine + methyl iodide
-
 ?
2.1.1.165 S-adenosyl-L-methionine + iodide
-
 Papenfussiella kuromo S-adenosyl-L-homocysteine + methyl iodide
-
 ?
2.1.1.165 S-adenosyl-L-methionine + iodide
-
 Sargassum horneri S-adenosyl-L-homocysteine + methyl iodide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.165 SAM:halide ion methyltransferase
-
 Pavlova gyrans
2.1.1.165 SAM:halide ion methyltransferase
-
 Papenfussiella kuromo
2.1.1.165 SAM:halide ion methyltransferase
-
 Sargassum horneri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.165 30
-
 assay at Pavlova gyrans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.165 7 7.5
-
 Pavlova gyrans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.165 6 8 about 60% of maximal activity at pH 6.0 and 8.0 Pavlova gyrans