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Literature summary extracted from

  • Escamilla-Trevino, L.L.; Shen, H.; Uppalapati, S.R.; Ray, T.; Tang, Y.; Hernandez, T.; Yin, Y.; Xu, Y.; Dixon, R.A.
    Switchgrass (Panicum virgatum) possesses a divergent family of cinnamoyl CoA reductases with distinct biochemical properties (2009), New Phytol., 185, 143-155.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.44 heterologous expression as His-tagged fusion protein in Escherichia coli Panicum virgatum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.44 5-hydroxyferuloyl-CoA substrate inhibition at high concentration above 60 microM; substrate inhibition at high concentration above 60 microM Panicum virgatum
1.2.1.44 feruloyl-CoA substrate inhibition at high concentration above 60 microM; substrate inhibition at high concentration above 60 microM Panicum virgatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.44 0.0254
-
 feruloyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
1.2.1.44 0.0296
-
 caffeoyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
1.2.1.44 0.0316
-
 4-coumaroyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
1.2.1.44 0.0376
-
 caffeoyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
1.2.1.44 0.047
-
 feruloyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
1.2.1.44 0.0548
-
 sinapoyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
1.2.1.44 0.0565
-
 sinapoyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
1.2.1.44 0.0572
-
 4-coumaroyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.44 37880
-
 predicted Panicum virgatum
1.2.1.44 39040
-
 predicted Panicum virgatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.44 4-coumaroyl-CoA + NADPH + H+ Panicum virgatum
-
 4-coumaroylaldehyde + CoA + NADP+
-
 r
1.2.1.44 4-coumaroyl-CoA + NADPH + H+ Panicum virgatum preferenced substrate 4-coumaroylaldehyde + CoA + NADP+
-
 r
1.2.1.44 5-hydroxyferuloyl-CoA + NADPH + H+ Panicum virgatum
-
 5-hydroxyferulic aldehyde + CoA + NADP+
-
 r
1.2.1.44 caffeoyl-CoA + NADPH + H+ Panicum virgatum
-
 caffeolylaldehyde + CoA + NADP+
-
 r
1.2.1.44 caffeoyl-CoA + NADPH + H+ Panicum virgatum preferenced substrate caffeolylaldehyde + CoA + NADP+
-
 r
1.2.1.44 feruloyl-CoA + NADPH + H+ Panicum virgatum
-
 coniferaldehyde + CoA + NADP+
-
 r
1.2.1.44 feruloyl-CoA + NADPH + H+ Panicum virgatum preferred substrate coniferaldehyde + CoA + NADP+
-
 r
1.2.1.44 additional information Panicum virgatum based on its properties and expression pattern, PvCCR1 is probably associated with lignin biosynthesis during plant development and is therefore a target for the engineering of improved biomass, whereas PvCCR2 may function in defense ?
-
 ?
1.2.1.44 additional information Panicum virgatum PvCCR1 is probably associated with lignin biosynthesis during plant development ?
-
 ?
1.2.1.44 sinapoyl-CoA + NADPH + H+ Panicum virgatum very low efficiency sinapaldehyde + CoA + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.44 Panicum virgatum D2IX40
-
-
1.2.1.44 Panicum virgatum D2IX45
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.44 as His-tagged enzyme by means of Ni-NTA beads Panicum virgatum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.44 internode
-
 Panicum virgatum
-
1.2.1.44 leaf
-
 Panicum virgatum
-
1.2.1.44 node
-
 Panicum virgatum
-
1.2.1.44 seedling Alamo cultivar Panicum virgatum
-
1.2.1.44 stem
-
 Panicum virgatum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.44 4-coumaroyl-CoA + NADPH + H+
-
 Panicum virgatum 4-coumaroylaldehyde + CoA + NADP+
-
 r
1.2.1.44 4-coumaroyl-CoA + NADPH + H+ preferenced substrate Panicum virgatum 4-coumaroylaldehyde + CoA + NADP+
-
 r
1.2.1.44 5-hydroxyferuloyl-CoA + NADPH + H+
-
 Panicum virgatum 5-hydroxyferulic aldehyde + CoA + NADP+
-
 r
1.2.1.44 caffeoyl-CoA + NADPH + H+
-
 Panicum virgatum caffeolylaldehyde + CoA + NADP+
-
 r
1.2.1.44 caffeoyl-CoA + NADPH + H+ preferenced substrate Panicum virgatum caffeolylaldehyde + CoA + NADP+
-
 r
1.2.1.44 feruloyl-CoA + NADPH + H+
-
 Panicum virgatum coniferaldehyde + CoA + NADP+
-
 r
1.2.1.44 feruloyl-CoA + NADPH + H+ preferred substrate Panicum virgatum coniferaldehyde + CoA + NADP+
-
 r
1.2.1.44 additional information based on its properties and expression pattern, PvCCR1 is probably associated with lignin biosynthesis during plant development and is therefore a target for the engineering of improved biomass, whereas PvCCR2 may function in defense Panicum virgatum ?
-
 ?
1.2.1.44 additional information PvCCR1 is probably associated with lignin biosynthesis during plant development Panicum virgatum ?
-
 ?
1.2.1.44 sinapoyl-CoA + NADPH + H+ very low efficiency Panicum virgatum sinapaldehyde + CoA + NADP+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.2.1.44 CCR ambiguous Panicum virgatum
1.2.1.44 cinnamoyl CoA reductase
-
 Panicum virgatum
1.2.1.44 PvCCR1
-
 Panicum virgatum
1.2.1.44 PvCCR2
-
 Panicum virgatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.44 30
-
 assay at Panicum virgatum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.44 0.013
-
 sinapoyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
1.2.1.44 0.063
-
 feruloyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
1.2.1.44 0.162
-
 caffeoyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
1.2.1.44 0.188
-
 4-coumaroyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
1.2.1.44 0.2
-
 sinapoyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
1.2.1.44 0.23
-
 caffeoyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
1.2.1.44 0.32
-
 4-coumaroyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum
1.2.1.44 2.01
-
 feruloyl-CoA 100 mM sodium/potassium phosphate buffer pH 6.3, 250 mM NADPH Panicum virgatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.44 6.3
-
 assay at Panicum virgatum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.1.44 Panicum virgatum predicted
-
 6
1.2.1.44 Panicum virgatum predicted
-
 6.6