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Literature summary extracted from

  • Bublin, M.; Radauer, C.; Knulst, A.; Wagner, S.; Scheiner, O.; Mackie, A.R.; Mills, E.N.; Breiteneder, H.
    Effects of gastrointestinal digestion and heating on the allergenicity of the kiwi allergens Act d 1, actinidin, and Act d 2, a thaumatin-like protein (2008), Mol. Nutr. Food Res., 52, 1130-1139.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.14 additional information Actinidia deliciosa digestibility of kiwifruit allergens, actinidin (Act d 1) and thaumatin-like protein (Act d 2), is assessed using an in vitro digestion system that approximates physiological conditions with respect to the passage of food through the stomach into the duodenum: Act d 1 precipitates in simulated gastric fluid at pH 2 and digestion of the aggregated protein proceeds slowly. The residual precipitate redissolves completely in simulated duodenal fluid at pH 6.5 and is partially digested. Act d 1 and Act d 2 display nearly unchanged IgE binding abilities ?
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Organism

EC Number Organism UniProt Comment Textmining
3.4.22.14 Actinidia deliciosa
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.14 Act d 1 is purified from kiwifruit extracts by covalent chromatography using activated thiopropyl sepharose 6B Actinidia deliciosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.14 additional information digestibility of kiwifruit allergens, actinidin (Act d 1) and thaumatin-like protein (Act d 2), is assessed using an in vitro digestion system that approximates physiological conditions with respect to the passage of food through the stomach into the duodenum: Act d 1 precipitates in simulated gastric fluid at pH 2 and digestion of the aggregated protein proceeds slowly. The residual precipitate redissolves completely in simulated duodenal fluid at pH 6.5 and is partially digested. Act d 1 and Act d 2 display nearly unchanged IgE binding abilities Actinidia deliciosa ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.14 Act d 1
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 Actinidia deliciosa
3.4.22.14 actinidin
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 Actinidia deliciosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.14 additional information
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 thermal stability of Act d 1 and Act d 2 is strongly pH dependent. Act d 1 is irreversibly destabilized in acidic solutions (pH 3.5) Actinidia deliciosa