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Literature summary extracted from
- In vitro characterization of a carotenoid cleavage dioxygenase from Nostoc sp. PCC 7120 reveals a novel cleavage pattern, cytosolic localization and induction by highlight (2008), Mol. Microbiol., 69, 231-244.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.51 | additional information | induction of enzyme activity with highlight treatment | Nostoc sp. PCC 7120 = FACHB-418 |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.11.51 | expression as a glutathion-S-transferase fusion protein in Escherichia coli BL21 | Nostoc sp. PCC 7120 = FACHB-418 |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.51 | additional information | decreased enzyme activtiy at lower temperature | Nostoc sp. PCC 7120 = FACHB-418 |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.13.11.51 | cytosol | - |
Nostoc sp. PCC 7120 = FACHB-418 | 5829 | - |
| 1.13.11.51 | soluble | - |
Nostoc sp. PCC 7120 = FACHB-418 | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.51 | 55000 | - |
value about, Western blot | Nostoc sp. PCC 7120 = FACHB-418 |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.51 | Nostoc sp. PCC 7120 = FACHB-418 | Q8YXV3 | Anabaena sp. PCC 7120 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.51 | - |
Nostoc sp. PCC 7120 = FACHB-418 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.51 | 3-OH-beta-apo-10'-carotenal + O2 | assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone | Nostoc sp. PCC 7120 = FACHB-418 | apo-10',10-apocarotene-dial + ? | - |
? |  |
| 1.13.11.51 | 3-OH-beta-apo-8'-carotenal + O2 | assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone | Nostoc sp. PCC 7120 = FACHB-418 | apo-8',10-apocarotene-dial | - |
? | |
| 1.13.11.51 | 4-hydroxymyxol 2'-fucoside + O2 | assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone | Nostoc sp. PCC 7120 = FACHB-418 | ? | - |
? | |
| 1.13.11.51 | 4-ketomyxol 2'-fucoside + O2 | assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone | Nostoc sp. PCC 7120 = FACHB-418 | ? | - |
? | |
| 1.13.11.51 | astaxanthin + O2 | assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone | Nostoc sp. PCC 7120 = FACHB-418 | ? | - |
? | |
| 1.13.11.51 | beta-apo-10'-carotenal + O2 | assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone | Nostoc sp. PCC 7120 = FACHB-418 | ? | - |
? | |
| 1.13.11.51 | beta-apo-8'-carotenal + O2 | assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone | Nostoc sp. PCC 7120 = FACHB-418 | ? | - |
? | |
| 1.13.11.51 | canthaxanthin + O2 | assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone | Nostoc sp. PCC 7120 = FACHB-418 | ? | - |
? | |
| 1.13.11.51 | echinenone + O2 | assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone | Nostoc sp. PCC 7120 = FACHB-418 | ? | - |
? | |
| 1.13.11.51 | gamma-carotene + O2 | assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone | Nostoc sp. PCC 7120 = FACHB-418 | ? | - |
? | |
| 1.13.11.51 | myxol + O2 | assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone | Nostoc sp. PCC 7120 = FACHB-418 | ? | - |
? | |
| 1.13.11.51 | myxol 2'-fucoside + O2 | assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone | Nostoc sp. PCC 7120 = FACHB-418 | ? | - |
? | |
| 1.13.11.51 | zeaxanthin + O2 | assay at pH 7.8, 28°C, incubation stopped by adding one volume of acetone | Nostoc sp. PCC 7120 = FACHB-418 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.51 | carotenoid cleavage dioxygenase | - |
Nostoc sp. PCC 7120 = FACHB-418 |
| 1.13.11.51 | NosCCD | - |
Nostoc sp. PCC 7120 = FACHB-418 |
| 1.13.11.51 | Nostoc carotenoid cleavage dioxygenase | - |
Nostoc sp. PCC 7120 = FACHB-418 |
| 1.13.11.51 | NSC1 | - |
Nostoc sp. PCC 7120 = FACHB-418 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.51 | 28 | - |
at 20°C decreased enzyme acitivity | Nostoc sp. PCC 7120 = FACHB-418 |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.51 | 7.8 | - |
assay at | Nostoc sp. PCC 7120 = FACHB-418 |
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