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Literature summary extracted from

  • Zaar, A.; Gescher, J.; Eisenreich, W.; Bacher, A.; Fuchs, G.
    New enzymes involved in aerobic benzoate metabolism in Azoarcus evansii (2004), Mol. Microbiol., 54, 223-238.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.208 BoxC addition of the putative ring cleaving enzyme BoxC leads to a several-fold acceleration of the initial rate and completes conversion of benzoyl-CoA. BoxC might facilitate the binding of BoxA to BoxB and thus lead to the observed rate increase Aromatoleum evansii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.208 KCl higher salt concentration (500 mM KCl) results in sixfold lower activity suggesting that the interaction of the protein components is affected by high salt concentration Aromatoleum evansii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.208 0.03
-
 benzoyl-CoA pH 8, 22°C, addition of the putative ring cleaving enzyme BoxC leads to a several-fold acceleration of the initial rate and completes conversion of benzoyl-CoA. Because of this complex behaviour of the oxygenase system, the apparent Km value for benzoyl-CoA can only be estimated from the time curve of benzoyl-CoA concentration in an assay mixture that contains BoxAB and the putative ring-cleaving enzyme BoxC. This curve shows a half-maximal rate at a benzoyl-CoA concentration of 0.03 mM Aromatoleum evansii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.208 Fe BoxA is a homodimeric 46 kDa iron-sulfur-flavoprotein, which acts as reductase. BoxB is a monomeric iron-protein. Subunit BoxB contains 1.6 mol of iron per mol of protein, addition of 1 mM Fe2+ to the assays has no stimulatory or inhibitory effect on the catalytic turnover Aromatoleum evansii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.208 46000
-
 2 * 46000 (BoxA) + 50000 (BoxB), boxA/boxB system, BoxA is a homodimeric 46 kDa iron-sulfur-flavoprotein, which acts as reductase. BoxB is a monomeric iron-protein (50000-60000 Da, gel filtration. 50000 Da, SDS-PAGE) Aromatoleum evansii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.208 benzoyl-CoA + NADPH + H+ + O2 Aromatoleum evansii the enzyme is involved in aerobic benzoate metabolism in Azoarcus evansii 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + NADP+
-
 ?
1.14.13.208 benzoyl-CoA + NADPH + H+ + O2 Aromatoleum evansii KB740 the enzyme is involved in aerobic benzoate metabolism in Azoarcus evansii 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.208 Aromatoleum evansii Q9AIX6 and Q9AIX7 Q9AIX6: subunit A, Q9AIX7: subunit B
-
1.14.13.208 Aromatoleum evansii KB740 Q9AIX6 and Q9AIX7 Q9AIX6: subunit A, Q9AIX7: subunit B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.208 His6-tagged component B of benzoyl-CoA oxygenase, component A of benzoyl-CoA oxygenase Aromatoleum evansii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.208 2-fluorobenzoyl-CoA + NADPH + H+ + O2 83% of the activity with benzoyl-CoA Aromatoleum evansii ?
-
 ?
1.14.13.208 2-fluorobenzoyl-CoA + NADPH + H+ + O2 83% of the activity with benzoyl-CoA Aromatoleum evansii KB740 ?
-
 ?
1.14.13.208 4-fluorobenzoyl-CoA + NADPH + H+ + O2 30% of the activity with benzoyl-CoA Aromatoleum evansii ?
-
 ?
1.14.13.208 4-fluorobenzoyl-CoA + NADPH + H+ + O2 30% of the activity with benzoyl-CoA Aromatoleum evansii KB740 ?
-
 ?
1.14.13.208 benzoyl-CoA + NADPH + H+ + O2 the enzyme is involved in aerobic benzoate metabolism in Azoarcus evansii Aromatoleum evansii 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + NADP+
-
 ?
1.14.13.208 benzoyl-CoA + NADPH + H+ + O2 no activity with NADH as electron donor Aromatoleum evansii 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + NADP+
-
 ?
1.14.13.208 benzoyl-CoA + NADPH + H+ + O2 the enzyme is involved in aerobic benzoate metabolism in Azoarcus evansii Aromatoleum evansii KB740 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + NADP+
-
 ?
1.14.13.208 benzoyl-CoA + NADPH + H+ + O2 no activity with NADH as electron donor Aromatoleum evansii KB740 2,3-dihydro-2,3-dihydroxybenzoyl-CoA + NADP+
-
 ?
1.14.13.208 additional information no activity with benzoate or phenylacetyl-CoA Aromatoleum evansii ?
-
 ?
1.14.13.208 additional information no activity with benzoate or phenylacetyl-CoA Aromatoleum evansii KB740 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.208 ? 2 * 46000 (BoxA) + 50000 (BoxB), boxA/boxB system, BoxA is a homodimeric 46 kDa iron-sulfur-flavoprotein, which acts as reductase. BoxB is a monomeric iron-protein (50000-60000 Da, gel filtration. 50000 Da, SDS-PAGE) Aromatoleum evansii

Synonyms

EC Number Synonyms Comment Organism
1.14.13.208 benzoyl-CoA dioxygenase/reductase
-
 Aromatoleum evansii
1.14.13.208 BoxA/BoxB system
-
 Aromatoleum evansii
1.14.13.208 BoxBA
-
 Aromatoleum evansii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.208 22
-
 assay at Aromatoleum evansii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.208 8
-
-
 Aromatoleum evansii

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.208 NADPH no activity with NADH Aromatoleum evansii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.13.208 Aromatoleum evansii calculated from sequence
-
 5.6