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Literature summary extracted from
- Molecular cloning and expression analysis of a cytokinin oxidase (DhCKX) gene in Dendrobium huoshanense (2008), Mol. Biol. Rep., 36, 1331-1338.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.99.12 | additional information | expression of the DhCKX gene is induced in roots, shoot apices and leaves, but not in stems, after treatment with 0.02 mM 6-benzylamino-purine | Dendrobium huoshanense |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.5.99.12 | - |
Dendrobium huoshanense |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.5.99.12 | 60383 | - |
x * 60383, calculated from the deduced amino acid sequence | Dendrobium huoshanense |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.99.12 | additional information | Dendrobium huoshanense | irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde | ? | - |
? |  |
| 1.5.99.12 | N6-isopentenyladenine + electron acceptor | Dendrobium huoshanense | - |
adenine + 3-methyl-2-butenal + reduced electron acceptor | - |
ir | |
| 1.5.99.12 | zeatin + electron acceptor | Dendrobium huoshanense | - |
4-hydroxy-3-methylbut-2-enal + adenine + reduced electron acceptor | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.99.12 | Dendrobium huoshanense | A2TLJ3 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.5.99.12 | leaf | low level | Dendrobium huoshanense | - |
| 1.5.99.12 | additional information | not detectable in stem | Dendrobium huoshanense | - |
| 1.5.99.12 | root | high level | Dendrobium huoshanense | - |
| 1.5.99.12 | shoot | high level in shoot apices | Dendrobium huoshanense | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.99.12 | additional information | irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde | Dendrobium huoshanense | ? | - |
? | |
| 1.5.99.12 | N6-isopentenyladenine + electron acceptor | - |
Dendrobium huoshanense | adenine + 3-methyl-2-butenal + reduced electron acceptor | - |
ir | |
| 1.5.99.12 | zeatin + electron acceptor | - |
Dendrobium huoshanense | 4-hydroxy-3-methylbut-2-enal + adenine + reduced electron acceptor | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.99.12 | ? | x * 60383, calculated from the deduced amino acid sequence | Dendrobium huoshanense |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.5.99.12 | CKX | - |
Dendrobium huoshanense |
| 1.5.99.12 | DhCKX | - |
Dendrobium huoshanense |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.99.12 | FAD | - |
Dendrobium huoshanense | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.5.99.12 | Dendrobium huoshanense | calculated from the deduced amino acid sequence | - |
6.6 |
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