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Literature summary extracted from

  • Rothacker, B.; Werr, M.; Ilg, T.
    Succinic semialdehyde dehydrogenase from the parasitic cattle tick Rhipicephalus microplus: gene identification, biochemical characterization and comparison with the mouse ortholog (2008), Mol. Biochem. Parasitol., 161, 32-43.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.24 expressed in Escherichia coli M15 cells Rhipicephalus microplus
1.2.1.24 expressed in Escherichia coli M15 cells Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.24 4-hydroxybenzaldehyde more than 95% inhibition at 0.05 mM Mus musculus
1.2.1.24 4-hydroxybenzaldehyde more than 95% inhibition at 0.05 mM Rhipicephalus microplus
1.2.1.24 4-methoxybenzaldehyde
-
 Mus musculus
1.2.1.24 4-methoxybenzaldehyde
-
 Rhipicephalus microplus
1.2.1.24 4-tolualdehyde
-
 Mus musculus
1.2.1.24 4-tolualdehyde
-
 Rhipicephalus microplus
1.2.1.24 succinate semialdehyde the enzyme loses more than 80% of its enzymatic potential at 0.5 mM and shows 8% residual activity at 3 mM succinate semialdehyde Mus musculus
1.2.1.24 succinate semialdehyde the enzyme loses more than 80% of its enzymatic potential at 0.5 mM and shows 8% residual activity at 3 mM succinate semialdehyde Rhipicephalus microplus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.24 0.00247
-
 succinate semialdehyde in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Mus musculus
1.2.1.24 0.00256
-
 succinate semialdehyde in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Rhipicephalus microplus
1.2.1.24 0.0151
-
 4-carboxybenzaldehyde in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Mus musculus
1.2.1.24 0.0253
-
 n-hexanal in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Mus musculus
1.2.1.24 0.0287
-
 n-Pentanal in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Mus musculus
1.2.1.24 0.0312
-
 4-carboxybenzaldehyde in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Rhipicephalus microplus
1.2.1.24 0.0331
-
 3-carboxybenzaldehyde in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Mus musculus
1.2.1.24 0.0433
-
 NAD+ in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Rhipicephalus microplus
1.2.1.24 0.0502
-
 3-Nitrobenzaldehyde in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Rhipicephalus microplus
1.2.1.24 0.0514
-
 n-octanal in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Rhipicephalus microplus
1.2.1.24 0.061
-
 n-Butanal in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Mus musculus
1.2.1.24 0.096
-
 n-Pentanal in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Rhipicephalus microplus
1.2.1.24 0.1035
-
 3-(methoxycarbonyl)benzoate in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Rhipicephalus microplus
1.2.1.24 0.107
-
 n-hexanal in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Rhipicephalus microplus
1.2.1.24 0.115
-
 4-carboxybenzaldehyde in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Mus musculus
1.2.1.24 0.137
-
 n-octanal in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Mus musculus
1.2.1.24 0.1418
-
 3-carboxybenzaldehyde in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Rhipicephalus microplus
1.2.1.24 0.156
-
 4-nitrobenzaldehyde in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Mus musculus
1.2.1.24 0.1612
-
 4-nitrobenzaldehyde in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Rhipicephalus microplus
1.2.1.24 0.1668
-
 4-carboxybenzaldehyde in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Rhipicephalus microplus
1.2.1.24 0.167
-
 benzaldehyde in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Rhipicephalus microplus
1.2.1.24 0.218
-
 methyl 3-formylbenzoate in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Mus musculus
1.2.1.24 0.227
-
 NAD+ in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Mus musculus
1.2.1.24 0.287
-
 3-Nitrobenzaldehyde in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Mus musculus
1.2.1.24 0.323
-
 n-Butanal in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Rhipicephalus microplus
1.2.1.24 0.616
-
 NADP+ in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Mus musculus
1.2.1.24 1.212
-
 propanal in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Mus musculus
1.2.1.24 1.622
-
 propanal in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Rhipicephalus microplus
1.2.1.24 2.797
-
 acetaldehyde in 100 mM Na2HPO4/NaH2PO4 buffer, 1 mM dithiothreitol, at pH 8.5 and 22°C Rhipicephalus microplus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.24 98000
-
 SDS-PAGE Rhipicephalus microplus
1.2.1.24 98000
-
 SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.24 Mus musculus Q8BWF0
-
-
1.2.1.24 Rhipicephalus microplus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.24 Ni2+-NTA agarose column chromatography Rhipicephalus microplus
1.2.1.24 Ni2+-NTA agarose column chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.24 brain
-
 Mus musculus
-
1.2.1.24 larva
-
 Rhipicephalus microplus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.24 2-carboxybenzaldehyde + NAD+ + H2O about 1% activity compared to succinate semialdehyde Mus musculus 2-carboxybenzoate + NADH + H+
-
 ?
1.2.1.24 2-hydroxybenzaldehyde + NAD+ + H2O about 1% activity compared to succinate semialdehyde Mus musculus 2-hydroxybenzoate + NADH + H+
-
 ?
1.2.1.24 2-nitrobenzaldehyde + NAD+ + H2O about 3% activity compared to succinate semialdehyde Mus musculus 2-nitrobenzoate + NADH + H+
-
 ?
1.2.1.24 2-phenylacetaldehyde + NAD+ + H2O about 5% activity compared to succinate semialdehyde Mus musculus 2-phenylbenzoate + NADH + H+
-
 ?
1.2.1.24 2-tolualdehyde + NAD+ + H2O about 1% activity compared to succinate semialdehyde Mus musculus 2-carboxytoluene + NADH + H+
-
 ?
1.2.1.24 3-carboxybenzaldehyde + NAD+ + H2O about 20% activity compared to succinate semialdehyde Mus musculus 3-carboxybenzoate + NADH + H+
-
 ?
1.2.1.24 3-carboxybenzaldehyde + NAD+ + H2O about 35% activity compared to succinate semialdehyde Rhipicephalus microplus 3-carboxybenzoate + NADH + H+
-
 ?
1.2.1.24 3-hydroxybenzaldehyde + NAD+ + H2O about 1% activity compared to succinate semialdehyde Mus musculus 3-hydroxybenzoate + NADH + H+
-
 ?
1.2.1.24 3-hydroxybenzaldehyde + NAD+ + H2O about 2% activity compared to succinate semialdehyde Mus musculus 3-hydroxybenzoate + NADH + H+
-
 ?
1.2.1.24 3-hydroxybenzaldehyde + NAD+ + H2O about 3% activity compared to succinate semialdehyde Rhipicephalus microplus 3-hydroxybenzoate + NADH + H+
-
 ?
1.2.1.24 3-methoxy-benzaldehyde + NAD+ + H2O about 10% activity compared to succinate semialdehyde Rhipicephalus microplus 3-methoxy-benzoate + NADH + H+
-
 ?
1.2.1.24 3-methoxy-benzaldehyde + NAD+ + H2O about 5% activity compared to succinate semialdehyde Mus musculus 3-methoxy-benzoate + NADH + H+
-
 ?
1.2.1.24 3-methylbenzaldehyde + NAD+ + H2O about 10% activity compared to succinate semialdehyde Rhipicephalus microplus 3-methylbenzoate + NADH + H+
-
 ?
1.2.1.24 3-methylbenzaldehyde + NAD+ + H2O about 3% activity compared to succinate semialdehyde Mus musculus 3-methylbenzoate + NADH + H+
-
 ?
1.2.1.24 3-nitrobenzaldehyde + NAD+ + H2O about 15% activity compared to succinate semialdehyde Rhipicephalus microplus 3-nitrobenzoate + NADH + H+
-
 ?
1.2.1.24 3-nitrobenzaldehyde + NAD+ + H2O about 20% activity compared to succinate semialdehyde Mus musculus 3-nitrobenzoate + NADH + H+
-
 ?
1.2.1.24 4-carboxybenzaldehyde + NAD+ + H2O about 10% activity compared to succinate semialdehyde Mus musculus 4-carboxybenzoate + NADH + H+
-
 ?
1.2.1.24 4-carboxybenzaldehyde + NAD+ + H2O about 65% activity compared to succinate semialdehyde Rhipicephalus microplus 4-carboxybenzoate + NADH + H+
-
 ?
1.2.1.24 4-methoxy-benzaldehyde + NAD+ + H2O about 1% activity compared to succinate semialdehyde Mus musculus 4-methoxy-benzoate + NADH + H+
-
 ?
1.2.1.24 4-methoxy-benzaldehyde + NAD+ + H2O about 5% activity compared to succinate semialdehyde Rhipicephalus microplus 4-methoxy-benzoate + NADH + H+
-
 ?
1.2.1.24 4-nitrobenzaldehyde + NAD+ + H2O about 15% activity compared to succinate semialdehyde Mus musculus 4-nitrobenzoate + NADH + H+
-
 ?
1.2.1.24 4-nitrobenzaldehyde + NAD+ + H2O about 30% activity compared to succinate semialdehyde Rhipicephalus microplus 4-nitrobenzoate + NADH + H+
-
 ?
1.2.1.24 acetaldehyde + NAD+ + H2O about 10% activity compared to succinate semialdehyde Rhipicephalus microplus acetate + NADH + H+
-
 ?
1.2.1.24 acetaldehyde + NAD+ + H2O about 5% activity compared to succinate semialdehyde Mus musculus acetate + NADH + H+
-
 ?
1.2.1.24 benzaldehyde + NAD+ + H2O about 30% activity compared to succinate semialdehyde Rhipicephalus microplus benzoate + NADH + H+
-
 ?
1.2.1.24 benzaldehyde + NAD+ + H2O about 5% activity compared to succinate semialdehyde Mus musculus benzoate + NADH + H+
-
 ?
1.2.1.24 glyoxylic acid + NAD+ + H2O about 1% activity compared to succinate semialdehyde Mus musculus 2-phenylbenzoate + NADH + H+
-
 ?
1.2.1.24 iso-butanal + NAD+ + H2O less than 10% activity compared to succinate semialdehyde Mus musculus ? + NADH + H+
-
 ?
1.2.1.24 iso-butanal + NAD+ + H2O less than 10% activity compared to succinate semialdehyde Rhipicephalus microplus isobutanoate + NADH + H+
-
 ?
1.2.1.24 iso-pentanal + NAD+ + H2O about 2% activity compared to succinate semialdehyde Mus musculus ? + NADH + H+
-
 ?
1.2.1.24 iso-pentanal + NAD+ + H2O less than 5% activity compared to succinate semialdehyde Rhipicephalus microplus isopentanoate + NADH + H+
-
 ?
1.2.1.24 methyl 3-formylbenzoate + NAD+ + H2O about 20% activity compared to succinate semialdehyde Mus musculus 3-(methoxycarbonyl)benzoate + NADH + H+
-
 ?
1.2.1.24 methyl 3-formylbenzoate + NAD+ + H2O about 45% activity compared to succinate semialdehyde Rhipicephalus microplus 3-(methoxycarbonyl)benzoate + NADH + H+
-
 ?
1.2.1.24 methyl 4-formylbenzoate + NAD+ + H2O about 15% activity compared to succinate semialdehyde Mus musculus 4-(methoxycarbonyl)benzoate + NADH + H+
-
 ?
1.2.1.24 methyl 4-formylbenzoate + NAD+ + H2O about 30% activity compared to succinate semialdehyde Rhipicephalus microplus 4-(methoxycarbonyl)benzoate + NADH + H+
-
 ?
1.2.1.24 additional information no activity with NADP+ Rhipicephalus microplus ?
-
 ?
1.2.1.24 additional information no activity towards 2-methoxybenzaldehyde, 2-nitrobenzaldehyde, 2-carboxybenzaldehyde, 2-tolualdehyde, 4-tolualdehyde, 2-hydroxybenzaldehyde, 4-hydroxybenzaldehyde, 2-phenylacetaldedyde, and glyoxylic acid Rhipicephalus microplus ?
-
 ?
1.2.1.24 additional information no activity towards 2-methoxybenzaldehyde, and 4-tolualdehyde Mus musculus ?
-
 ?
1.2.1.24 n-butanal + NAD+ + H2O about 10% activity compared to succinate semialdehyde Mus musculus butanoate + NADH + H+
-
 ?
1.2.1.24 n-butanal + NAD+ + H2O less than 60% activity compared to succinate semialdehyde Rhipicephalus microplus butanoate + NADH + H+
-
 ?
1.2.1.24 n-hexanal + NAD+ + H2O about 110% activity compared to succinate semialdehyde Rhipicephalus microplus hexanoate + NADH + H+
-
 ?
1.2.1.24 n-hexanal + NAD+ + H2O less than 10% activity compared to succinate semialdehyde Mus musculus hexanoate + NADH + H+
-
 ?
1.2.1.24 n-octanal + NAD+ + H2O less than 10% activity compared to succinate semialdehyde Mus musculus n-octanoate + NADH + H+
-
 ?
1.2.1.24 n-octanal + NAD+ + H2O less than 80% activity compared to succinate semialdehyde Rhipicephalus microplus n-octanoate + NADH + H+
-
 ?
1.2.1.24 n-pentanal + NAD+ + H2O 100% activity Rhipicephalus microplus n-pentanoate + NADH + H+
-
 ?
1.2.1.24 n-pentanal + NAD+ + H2O less than 20% activity compared to succinate semialdehyde Mus musculus n-pentanoate + NADH + H+
-
 ?
1.2.1.24 propanal + NAD+ + H2O less than 10% activity compared to succinate semialdehyde Mus musculus propanoate + NADH + H+
-
 ?
1.2.1.24 propanal + NAD+ + H2O less than 20% activity compared to succinate semialdehyde Rhipicephalus microplus propanoate + NADH + H+
-
 ?
1.2.1.24 succinate semialdehyde + NAD+ + H2O 100% activity with succinate semialdehyde Rhipicephalus microplus succinate + NADH + H+
-
 ?
1.2.1.24 succinate semialdehyde + NAD+ + H2O 100% activity with succinate semialdehyde Mus musculus succinate + NADH + H+
-
 ?
1.2.1.24 succinate semialdehyde + NADP+ + H2O NAD+ is the more efficient coenzyme compared to NADP+, but the preference is not exclusive Mus musculus succinate + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.24 SSADH
-
 Rhipicephalus microplus
1.2.1.24 SSADH
-
 Mus musculus
1.2.1.24 succinic semialdehyde dehydrogenase
-
 Rhipicephalus microplus
1.2.1.24 succinic semialdehyde dehydrogenase
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.24 additional information no activity with NADP+ Rhipicephalus microplus
1.2.1.24 NAD+
-
 Rhipicephalus microplus
1.2.1.24 NAD+ NAD+ is the more efficient coenzyme compared to NADP+, but the preference is not exclusive Mus musculus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.2.1.24 0.1
-
-
 Rhipicephalus microplus 4-methoxybenzaldehyde
1.2.1.24 0.1
-
-
 Rhipicephalus microplus 4-tolualdehyde
1.2.1.24 1
-
-
 Mus musculus 4-tolualdehyde
1.2.1.24 2
-
-
 Mus musculus 4-methoxybenzaldehyde