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Literature summary extracted from

  • Smollett, K.L.; Fivian-Hughes, A.S.; Smith, J.E.; Chang, A.; Rao, T.; Davis, E.O.
    Experimental determination of translational start sites resolves uncertainties in genomic open reading frame predictions - application to Mycobacterium tuberculosis (2009), Microbiology, 155, 186-197.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.88 the lexA gene, including its promoter region, cloned into pEJMyc to give pKS04, giving LexA with an in-frame C-terminal Myc tag. Plasmids pKS04, pKS04mut1 (one residue deleted between the possible start codons) and the pEJMyc vector transformed separately into Mycobacterium smegmatis strain mc2155 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.88 27600
-
 Western blotting, Myc-tagged LexA Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.88 Mycobacterium tuberculosis P9WHR7
-
-
3.4.21.88 Mycobacterium tuberculosis H37Rv P9WHR7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.88 additional information presence of LexA-Myc is present in cell-free extract from Mycobacterium tuberculosis expressing pKS04 but not pKS04mut1 or pEJMyc. The translational start site for LexA is upstream of residue 1, it is translated from a start codon 57 bp further upstream at the transcriptional start site Mycobacterium tuberculosis ?
-
 ?
3.4.21.88 additional information presence of LexA-Myc is present in cell-free extract from Mycobacterium tuberculosis expressing pKS04 but not pKS04mut1 or pEJMyc. The translational start site for LexA is upstream of residue 1, it is translated from a start codon 57 bp further upstream at the transcriptional start site Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.88 LexA
-
 Mycobacterium tuberculosis