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Literature summary extracted from

  • Jeong, H.; Young, M.; Kim, B.; Ahn, J.
    Analysis of flavonoids and characterization of the OsFNS gene involved in flavone biosynthesis in rice (2008), J. Plant Biol., 51, 97-101.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.20.5 oxoglutarate no flavone synthase activity without this compound Oryza sativa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.20.5 RT-PCR cloning of flavone synthase cDNA, subcloning in pGEX 5X-2 for expression in Escherichia coli Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.20.5 0.02
-
 S-naringenin 37°C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate in 10 mM Tris/HCl buffer, pH 8.0 Oryza sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.20.5 FeSO4 65% reduced activity without FeSO4 Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.20.5 38700
-
 SDS-PAGE, 1027 bp, Fe2+-binding site, oxoglutarate binding site Oryza sativa
1.14.20.5 64700
-
 SDS-PAGE, flavone synthase + glutathione S-transferase (26000 Da) Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.20.5 S-naringenin + 2-oxoglutarate + O2 Oryza sativa purified enzyme, 3 h, 37°C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate (S- or R-naringenin) in 10 mM Tris/HCl buffer, pH 8.0 apigenin + succinate + CO2 + H2O apigenin is converted by falvonoid 3',5'-hydroxylase to luteolin and tricetin ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.5 Oryza sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.20.5 expression of flavone synthase in Escherichia coli, purification on glutathione S-transferase (GST) affinity column Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.20.5 leaf
-
 Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.20.5 S-naringenin + 2-oxoglutarate + O2 purified enzyme, 3 h, 37°C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate (S- or R-naringenin) in 10 mM Tris/HCl buffer, pH 8.0 Oryza sativa apigenin + succinate + CO2 + H2O apigenin is converted by falvonoid 3',5'-hydroxylase to luteolin and tricetin ?

Synonyms

EC Number Synonyms Comment Organism
1.14.20.5 flavone synthase
-
 Oryza sativa
1.14.20.5 FNS
-
 Oryza sativa
1.14.20.5 OsFNS
-
 Oryza sativa