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Literature summary extracted from
- Biochemical characterization of Rhodococcus erythropolis N'4 nitrile hydratase acting on 4-chloro-3-hydroxybutyronitrile (2008), J. Mol. Catal. B, 55, 157-163.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.2.1.84 | synthesis | enzyme can be used in conjunction with a stereoselective amidase to synthesize ethyl (S)-4-chloro-3-hydroxybutyrate, an intermediate for a hypercholesterolemia drug, Atorvastatin | Rhodococcus erythropolis |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.2.1.84 | the partially purified enzyme is stable in the presence of organic acids at higher temperatures | Rhodococcus erythropolis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.84 | 1,10-phenanthroline | 39% reduced activity at 1 mM | Rhodococcus erythropolis |  |
| 4.2.1.84 | 2-mercaptoethanol | 9% reduced activity at 1 mM | Rhodococcus erythropolis | |
| 4.2.1.84 | ammonium persulfate | 52% reduced activity at 1 mM | Rhodococcus erythropolis | |
| 4.2.1.84 | cobalt chloride | 20% reduced activity at 1 mM | Rhodococcus erythropolis | |
| 4.2.1.84 | copper sulfate | 95% reduced activity at 1 mM | Rhodococcus erythropolis | |
| 4.2.1.84 | dithiothreitol | 18% reduced activity at 1 mM | Rhodococcus erythropolis | |
| 4.2.1.84 | EDTA | 15% reduced activity at 1 mM | Rhodococcus erythropolis | |
| 4.2.1.84 | Ferrous sulfate | 54% reduced activity at 1 mM | Rhodococcus erythropolis | |
| 4.2.1.84 | hydrogen peroxide | 97% reduced activity at 1 mM | Rhodococcus erythropolis | |
| 4.2.1.84 | iodoacetamide | 22% reduced activity at 1 mM | Rhodococcus erythropolis | |
| 4.2.1.84 | Mercury chloride | 89% reduced activity at 1 mM | Rhodococcus erythropolis | |
| 4.2.1.84 | PMSF | 83% reduced activity at 1 mM | Rhodococcus erythropolis | |
| 4.2.1.84 | Silver nitrate | 82% reduced activity at 1 mM | Rhodococcus erythropolis | |
| 4.2.1.84 | zinc chloride | 56% reduced activity at 1 mM | Rhodococcus erythropolis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.84 | Fe3+ | NHase N'4 is a Fe-type enzyme with a Cys109-Ser110-Leu111-Cys112-Ser113-Cys114 sequence | Rhodococcus erythropolis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | 22953 | - |
2 * 27000, alpha-subunit, + 2 * 29000, beta-subunit, alpha2beta2 structure, SDS-PAGE, 2 * 22953, alpha-subunit, + 2 * 23486, beta-subunit, alpha2beta2 structure, sequence calculation | Rhodococcus erythropolis |
| 4.2.1.84 | 23486 | - |
2 * 27000, alpha-subunit, + 2 * 29000, beta-subunit, alpha2beta2 structure, SDS-PAGE, 2 * 22953, alpha-subunit, + 2 * 23486, beta-subunit, alpha2beta2 structure, sequence calculation | Rhodococcus erythropolis |
| 4.2.1.84 | 27000 | - |
2 * 27000, alpha-subunit, + 2 * 29000, beta-subunit, alpha2beta2 structure, SDS-PAGE, 2 * 22953, alpha-subunit, + 2 * 23486, beta-subunit, alpha2beta2 structure, sequence calculation | Rhodococcus erythropolis |
| 4.2.1.84 | 29000 | - |
2 * 27000, alpha-subunit, + 2 * 29000, beta-subunit, alpha2beta2 structure, SDS-PAGE, 2 * 22953, alpha-subunit, + 2 * 23486, beta-subunit, alpha2beta2 structure, sequence calculation | Rhodococcus erythropolis |
| 4.2.1.84 | 92300 | - |
gel filtration | Rhodococcus erythropolis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.84 | Rhodococcus erythropolis | - |
- |
- |
| 4.2.1.84 | Rhodococcus erythropolis N4 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.84 | native enzyme 78fold by ammonium sulfate fractionation, anion exchange chromatography, and hydrophobic interaction chromatography | Rhodococcus erythropolis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | 860 | - |
partially purified enzyme | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.84 | 4-chloro-3-hydroxybutyronitrile + H2O | the following reaction by an amidase leads to the correspondend carboxylic acid | Rhodococcus erythropolis | 4-chloro-3-hydroxybutyramide | - |
? | |
| 4.2.1.84 | acetonitrile + H2O | very low activity | Rhodococcus erythropolis | acetamide | - |
? | |
| 4.2.1.84 | acetonitrile + H2O | very low activity | Rhodococcus erythropolis N4 | acetamide | - |
? | |
| 4.2.1.84 | acrylonitrile + H2O | - |
Rhodococcus erythropolis | 2-propenoic acid amide | i.e. acrylamide | ? | |
| 4.2.1.84 | acrylonitrile + H2O | - |
Rhodococcus erythropolis N4 | 2-propenoic acid amide | i.e. acrylamide | ? | |
| 4.2.1.84 | benzonitrile + H2O | low activity | Rhodococcus erythropolis | benzoic acid amide | - |
? | |
| 4.2.1.84 | benzonitrile + H2O | low activity | Rhodococcus erythropolis N4 | benzoic acid amide | - |
? | |
| 4.2.1.84 | butyronitrile + H2O | best substrate | Rhodococcus erythropolis | butyric acid amide | - |
? | |
| 4.2.1.84 | isobutyronitrile + H2O | - |
Rhodococcus erythropolis | isobutyric acid amide | - |
? | |
| 4.2.1.84 | isobutyronitrile + H2O | - |
Rhodococcus erythropolis N4 | isobutyric acid amide | - |
? | |
| 4.2.1.84 | methacrylonitrile + H2O | - |
Rhodococcus erythropolis | methacrylic acid amide | - |
? | |
| 4.2.1.84 | propionitrile + H2O | - |
Rhodococcus erythropolis | propionic acid amide | - |
? | |
| 4.2.1.84 | propionitrile + H2O | - |
Rhodococcus erythropolis N4 | propionic acid amide | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.84 | tetramer | 2 * 27000, alpha-subunit, + 2 * 29000, beta-subunit, alpha2beta2 structure, SDS-PAGE, 2 * 22953, alpha-subunit, + 2 * 23486, beta-subunit, alpha2beta2 structure, sequence calculation | Rhodococcus erythropolis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.84 | NHase | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | 25 | - |
- |
Rhodococcus erythropolis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | 15 | 35 | 15°C: about 60% of maximal activity, 35°C: about 55% of maximal activity, temperature profile | Rhodococcus erythropolis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | 5 | 40 | 30 min, the purified enzyme is stable up to 25°C, inactivation at 40°C | Rhodococcus erythropolis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | 7.5 | - |
- |
Rhodococcus erythropolis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | 7 | 8.5 | pH 7.0: about 80% of maximal activity, pH 8.5: about 75% of maximal activity | Rhodococcus erythropolis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | 7 | 11 | 10°C, 30 min, purified enzyme, stable | Rhodococcus erythropolis |
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