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Literature summary extracted from

  • Gonzalez-Segura, L.; Rudino-Pinera, E.; Munoz-Clares, R.A.; Horjales, E.
    The crystal structure of a ternary complex of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa provides new insight into the reaction mechanism and shows a novel binding mode of the 2-phosphate of NADP+ and a novel cation binding site (2009), J. Mol. Biol., 385, 542-557.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.2.1.8 medicine the enzyme could be a target for antibiotic design Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.8 expressed in Escherichia coli Pseudomonas aeruginosa
1.2.1.8 plasmid pCALbetB containing the full sequence of the gene betB that encodes PaBADH is used for the expression of the enzyme in Escherichia coli cells Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.8 BADH in the presence of 2-mercaptoethanol, glycerol, NADP+ and 150 mM K+, hanging drop vapor diffusion method, using 85 mM HEPES–NaOH, pH 7.5, 8.5% (v/v) isopropanol, 17% (w/v) polyethylene glycol 4000 and 15% (v/v) glycerol, at 18°C Pseudomonas aeruginosa
1.2.1.8 the three dimensional structure of betaine aldehyde dehydrogenase, in complex with glycerol, NADP+ and K+ ions, is determined at 2.1 A resolution Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.8 K+
-
 Pseudomonas aeruginosa
1.2.1.8 K+ the enzyme requires K+ ions for stability and possesses two K+ binding sites per subunit Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD(P)+ + H2O Pseudomonas aeruginosa
-
 glycine betaine + NAD(P)H + H+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Pseudomonas aeruginosa
-
-
-
1.2.1.8 Pseudomonas aeruginosa Q9HTJ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.8
-
 Pseudomonas aeruginosa
1.2.1.8 by a two step procedure Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD(P)+ + H2O
-
 Pseudomonas aeruginosa glycine betaine + NAD(P)H + H+
-
 ir
1.2.1.8 betaine aldehyde + NAD+ + H2O
-
 Pseudomonas aeruginosa betaine + NADH + H+
-
 ir
1.2.1.8 betaine aldehyde + NADP+ + H2O
-
 Pseudomonas aeruginosa betaine + NADPH + H+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.2.1.8 homotetramer x-ray crystallography Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.2.1.8 aldehyde dehydrogenase
-
 Pseudomonas aeruginosa
1.2.1.8 BADH
-
 Pseudomonas aeruginosa
1.2.1.8 betaine aldehyde dehydrogenase
-
 Pseudomonas aeruginosa
1.2.1.8 betaine aldehyde: NAD(P)+ oxidoreductase
-
 Pseudomonas aeruginosa
1.2.1.8 PaBADH
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.8 NAD+ PaBADH uses NADP+ with the same efficiency as NAD+ Pseudomonas aeruginosa
1.2.1.8 NAD+ BADH uses NAD+ with the same efficiency as NADP+ Pseudomonas aeruginosa
1.2.1.8 NADP+ PaBADH uses NADP+ with the same efficiency as NAD+ Pseudomonas aeruginosa
1.2.1.8 NADP+ BADH uses NADP+ with the same efficiency as NAD+ Pseudomonas aeruginosa