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Literature summary extracted from

  • Jeoung, J.H.; Pippig, D.A.; Martins, B.M.; Wagener, N.; Dobbek, H.
    HTHP: a novel class of hexameric, tyrosine-coordinated heme proteins (2007), J. Mol. Biol., 368, 1122-1131.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.6 expressed in Escherichia coli Rosetta (DE3) Ruegeria pomeroyi DSS-3
1.11.1.7 expressed in Escherichia coli Rosetta (DE3) Ruegeria pomeroyi DSS-3

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.6
-
Ruegeria pomeroyi DSS-3
1.11.1.7
-
Ruegeria pomeroyi DSS-3

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 3-Amino-1,2,4-triazole 500 microM reduces the reactivity of catalase to 50% Ruegeria pomeroyi DSS-3
1.11.1.7 additional information peroxidase activity is not inhibited by 3-amino-1,2,4-triazole concentrations up to 20 mM Ruegeria pomeroyi DSS-3

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.6 17.2
-
H2O2
-
Ruegeria pomeroyi DSS-3

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 8300
-
ring-like arrangement of six monomers, 6 * 7900 (SDS-PAGE), 6 * 8300 calculated Ruegeria pomeroyi DSS-3
1.11.1.6 54000
-
gel filtration Ruegeria pomeroyi DSS-3
1.11.1.7 8300
-
ring-like arrangement of six monomers, 6 * 7900 (SDS-PAGE), 6 * 8300 calculated Ruegeria pomeroyi DSS-3
1.11.1.7 54000
-
gel filtration Ruegeria pomeroyi DSS-3

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Ruegeria pomeroyi DSS-3 Q5LLG6
-
-
1.11.1.7 Ruegeria pomeroyi DSS-3 Q5LLG6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 purified to homogeneity as holoprotein by affinity chromatography Ruegeria pomeroyi DSS-3
1.11.1.7 purified to homogeneity as holoprotein by affinity chromatography Ruegeria pomeroyi DSS-3

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.7 additional information
-
specific activity with o-dianisidine is 10fold lower at pH 7.5 and not measurable at higher pH values due to its instability in alkaline solution Ruegeria pomeroyi DSS-3

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 H2O2
-
Ruegeria pomeroyi DSS-3 O2 + H2O
-
?
1.11.1.7 additional information no peroxidase activity is observed with ascorbate as substrate Ruegeria pomeroyi DSS-3 ?
-
?
1.11.1.7 NADH + H2O2
-
Ruegeria pomeroyi DSS-3 NAD+ + H2O
-
?
1.11.1.7 NADPH + H2O2
-
Ruegeria pomeroyi DSS-3 NADP+ + H2O
-
?
1.11.1.7 o-dianisidine + H2O2
-
Ruegeria pomeroyi DSS-3 ? + H2O
-
?
1.11.1.7 pyrogallol + H2O2
-
Ruegeria pomeroyi DSS-3 purpurogallin + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 hexamer ring-like arrangement of six monomers, 6 * 7900 (SDS-PAGE), 6 * 8300 calculated Ruegeria pomeroyi DSS-3
1.11.1.7 hexamer ring-like arrangement of six monomers, 6 * 7900 (SDS-PAGE), 6 * 8300 calculated Ruegeria pomeroyi DSS-3

Synonyms

EC Number Synonyms Comment Organism
1.11.1.6 HTHP peroxidase and catalase activity Ruegeria pomeroyi DSS-3
1.11.1.6 tyrosine-coordinated heme protein
-
Ruegeria pomeroyi DSS-3
1.11.1.7 HTHP peroxidase and catalase activity Ruegeria pomeroyi DSS-3
1.11.1.7 tyrosine-coordinated heme protein
-
Ruegeria pomeroyi DSS-3

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.6 23
-
highest specific activity Ruegeria pomeroyi DSS-3
1.11.1.7 20
-
maximal activity Ruegeria pomeroyi DSS-3

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.6 additional information
-
high thermal stability. HTHP is stable under a wide range of temperatures. Thermal unfolding is measured up to 110°C and in the presence of high concentrations of guanidinium hydrochloride. The melting point of HTHP is estimated to be around 130°C. Catalatic activity is tested after incubation for 10 min at 85°C and 90°C and is found to be reduced by less than 5% under both conditions Ruegeria pomeroyi DSS-3
1.11.1.7 additional information
-
high thermal stability. HTHP is stable under a wide range of temperatures. Thermal unfolding is measured up to 110°C and in the presence of high concentrations of guanidinium hydrochloride. The melting point of HTHP is estimated to be around 130°C. Catalatic activity is tested after incubation for 10 min at 85°C and 90°C and is found to be reduced by less than 5% under both conditions Ruegeria pomeroyi DSS-3

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 10.5
-
highest specific activity Ruegeria pomeroyi DSS-3
1.11.1.7 8.7
-
at physiological pH values the highest activity is detectable with NADH or NADPH as substrates (3.0 U/mg at pH 8.7) Ruegeria pomeroyi DSS-3
1.11.1.7 11.7
-
highest peroxidase activity (25.7 U/mg), with pyrogallol as substrate Ruegeria pomeroyi DSS-3

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.6 additional information
-
HTHP is stable under a wide range of pH values Ruegeria pomeroyi DSS-3
1.11.1.7 additional information
-
high thermal stability. HTHP is stable under a wide range of pH Ruegeria pomeroyi DSS-3