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Literature summary extracted from

  • Zhang, F.; Qiao, D.; Xu, H.; Liao, C.; Li, S.; Cao, Y.
    Cloning, expression, and characterization of xylose reductase with higher activity from Candida tropicalis (2009), J. Microbiol., 47, 351-357.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.307 bovine serum albumin 1 mg/ml, 20% increase of activity Candida tropicalis
1.1.1.307 DTT 1 mM, 27% increase of activity Candida tropicalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.307 expressed in Escherichia coli BL21 (DE3), subloned into the pYES2 vector and transformed into Saccharomyces cerevisiae W303-1A Candida tropicalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.307 0.0455
-
 NADPH pH 5.5, 45°C Candida tropicalis
1.1.1.307 0.1619
-
 NADH pH 5.5, 45°C Candida tropicalis
1.1.1.307 31.5
-
 D-xylose pH 5.5, 45°C Candida tropicalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.307 additional information no increase in activity in presence of 1 mM NaCl and 1 mM MgSO4 Candida tropicalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.307 37100
-
 x * 37100, SDS-PAGE Candida tropicalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.307 D-xylose + NADH + H+ Candida tropicalis
-
 xylitol + NAD+
-
 ?
1.1.1.307 D-xylose + NADH + H+ Candida tropicalis SCTCC 300249
-
 xylitol + NAD+
-
 ?
1.1.1.307 D-xylose + NADPH + H+ Candida tropicalis key enzyme in xylose metabolism xylitol + NADP+
-
 ?
1.1.1.307 D-xylose + NADPH + H+ Candida tropicalis SCTCC 300249 key enzyme in xylose metabolism xylitol + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.307 Candida tropicalis A9QVV8 SCTCC 300249
-
1.1.1.307 Candida tropicalis SCTCC 300249 A9QVV8 SCTCC 300249
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.307 recombinant enzyme Candida tropicalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.307 251.5
-
 cofactor: NADPH Candida tropicalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.307 D-arabinose + NADPH + H+ about 60% of the activity compared to D-xylose Candida tropicalis ?
-
 ?
1.1.1.307 D-arabinose + NADPH + H+ about 60% of the activity compared to D-xylose Candida tropicalis SCTCC 300249 ?
-
 ?
1.1.1.307 D-galactose + NADPH + H+ about 70% of the activity compared to D-xylose Candida tropicalis ?
-
 ?
1.1.1.307 D-glucose + NADPH + H+ about 15% of the activity compared to D-xylose Candida tropicalis ?
-
 ?
1.1.1.307 D-ribose + NADPH + H+ about 90% of the activity compared to D-xylose Candida tropicalis ?
-
 ?
1.1.1.307 D-xylose + NADH + H+
-
 Candida tropicalis xylitol + NAD+
-
 ?
1.1.1.307 D-xylose + NADH + H+ dual coenzyme specificity, Km for NADPH: 0.0455 mM, Km for NADH: 0.162 mM Candida tropicalis xylitol + NAD+
-
 ?
1.1.1.307 D-xylose + NADH + H+
-
 Candida tropicalis SCTCC 300249 xylitol + NAD+
-
 ?
1.1.1.307 D-xylose + NADH + H+ dual coenzyme specificity, Km for NADPH: 0.0455 mM, Km for NADH: 0.162 mM Candida tropicalis SCTCC 300249 xylitol + NAD+
-
 ?
1.1.1.307 D-xylose + NADPH + H+ key enzyme in xylose metabolism Candida tropicalis xylitol + NADP+
-
 ?
1.1.1.307 D-xylose + NADPH + H+ dual coenzyme specificity, Km for NADPH: 0.0455 mM, Km for NADH: 0.162 mM Candida tropicalis xylitol + NADP+
-
 ?
1.1.1.307 D-xylose + NADPH + H+ key enzyme in xylose metabolism Candida tropicalis SCTCC 300249 xylitol + NADP+
-
 ?
1.1.1.307 D-xylose + NADPH + H+ dual coenzyme specificity, Km for NADPH: 0.0455 mM, Km for NADH: 0.162 mM Candida tropicalis SCTCC 300249 xylitol + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.307 ? x * 37100, SDS-PAGE Candida tropicalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.307 45
-
-
 Candida tropicalis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.307 30 60 30°C: about 70% of maximal activity, 60°C: about 45% of maximal activity Candida tropicalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.307 60
-
 1 h, 80% loss of activity, half-life is around 15 min Candida tropicalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.307 240
-
 D-xylose
-
 Candida tropicalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.307 5.5
-
-
 Candida tropicalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.307 4 6.5 pH 4.0: about 55% of maximal activity, pH 6.5: about 60% of maximal activity Candida tropicalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.307 NADH dual coenzyme specificity, Km for NADPH: 0.0455 mM, Km for NADH: 0.162 mM Candida tropicalis
1.1.1.307 NADPH dual coenzyme specificity, Km for NADPH: 0.0455 mM, Km for NADH: 0.162 mM Candida tropicalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.307 27430
-
 D-xylose pH 5.5, 45°C Candida tropicalis