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Literature summary extracted from
- Purification and characterization of thermostable beta-glucosidase from the brown-rot basidiomycete Fomitopsis palustris grown on microcrystalline cellulose (2008), J. Microbiol., 46, 51-55.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | D-gluconolactone | - |
Fomitopsis palustris |  |
| 3.2.1.21 | D-glucose | - |
Fomitopsis palustris | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.117 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.0, 50°C | Fomitopsis palustris | |
| 3.2.1.21 | 4.81 | - |
cellobiose | pH 5.0, 50°C | Fomitopsis palustris | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 138000 | - |
x * 138000, SDS-PAGE | Fomitopsis palustris |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Fomitopsis palustris | - |
growth on 2% microcrystalline cellulose | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | after growth on 2% microcrystalline cellulose | Fomitopsis palustris |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 191 | - |
pH 5.0, 50°C | Fomitopsis palustris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Fomitopsis palustris | 4-nitrophenol + D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Fomitopsis palustris | 2 D-glucose | - |
? | |
| 3.2.1.21 | additional information | very little or no activity against 4-nitrophenyl-beta-lactoside, 4-nitrophenyl-beta-xyloside, 4-nitrophenyl-alpha-arabinofuranoside, xylan, and carboxymethyl cellulose | Fomitopsis palustris | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | ? | x * 138000, SDS-PAGE | Fomitopsis palustris |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 70 | - |
- |
Fomitopsis palustris |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 55 | - |
half-life 97 h | Fomitopsis palustris |
| 3.2.1.21 | 65 | - |
hlaf-life 15 h | Fomitopsis palustris |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 101.8 | - |
cellobiose | - |
Fomitopsis palustris | |
| 3.2.1.21 | 721 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Fomitopsis palustris | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4.5 | - |
- |
Fomitopsis palustris |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.008 | - |
D-gluconolactone | pH 5.0, 50°C | Fomitopsis palustris | |
| 3.2.1.21 | 0.35 | - |
D-glucose | pH 5.0, 50°C | Fomitopsis palustris | |
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