EC Number | Application | Comment | Organism |
---|---|---|---|
1.14.20.5 | biotechnology | product of the combined activity of flavone synthase and flavone 7-O-methyltransferase produces genkwanin (7-O-methyl apigenin) which exhibits antibacterial activity against Vibrio cholerae and Enterococcus faecalis, and anti-inflammatory activity, direct plant extracts result in limited amounts | Populus deltoides |
2.1.1.B76 | synthesis | using Escherichia coli transformants harboring both PFNS-1 and POMT-7, naringenin could be converted into genkwanin | Populus sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.20.5 | the open reading frame of the flavone synthase gene PFNS-1 is cloned from Populus deltoids using RT-PCR, subcloned into Escherichia coli expression vector pGEX 5X-2, the flavone 7-O-methyltransferase into pRSF-Duet vector, both cotransformed into Escherichia coli BL21 | Populus deltoides |
2.1.1.B74 | gene POMT-7, recombinant expression of His-tagged enzyme in Escherichia coli | Populus deltoides |
2.1.1.B76 | using Escherichia coli transformants harboring both PFNS-1 and POMT-7, naringenin could be converted into genkwanin | Populus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.20.5 | 64000 | - |
SDS-PAGE, sum of flavone synthase and glutathione S-transferase (26000 Da) | Populus deltoides |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.20.5 | naringenin + O2 + 2-oxoglutarate | Populus deltoides | - |
apigenin + succinate + CO2 + H2O | flavone 7-O-methyltransferase (POMT-7) converts apigenin into 7-O-methyl apigenin (genkwanin), 28°C, 10 mM potassium phosphate buffer with 1% glucose, pH 6.0, 5 mM ascorbate, 10 microM FeSO4, 100 microM substrate naringenin, 15 h incubation | ? | |
2.1.1.B74 | S-adenosyl-L-methionine + apigenin | Populus deltoides | - |
S-adenosyl-L-homocysteine + 7-O-methylapigenin | i.e. genkwanin | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.20.5 | Populus deltoides | - |
- |
- |
2.1.1.B74 | Populus deltoides | - |
gene POMT-7 | - |
2.1.1.B76 | Populus sp. | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.20.5 | naringenin + O2 + 2-oxoglutarate | - |
Populus deltoides | apigenin + succinate + CO2 + H2O | flavone 7-O-methyltransferase (POMT-7) converts apigenin into 7-O-methyl apigenin (genkwanin), 28°C, 10 mM potassium phosphate buffer with 1% glucose, pH 6.0, 5 mM ascorbate, 10 microM FeSO4, 100 microM substrate naringenin, 15 h incubation | ? | |
2.1.1.B74 | S-adenosyl-L-methionine + apigenin | - |
Populus deltoides | S-adenosyl-L-homocysteine + 7-O-methylapigenin | i.e. genkwanin | ? | |
2.1.1.B76 | S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone | i.e. apigenin | Populus sp. | S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone | i.e. genkwanin | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.20.5 | flavone synthase | - |
Populus deltoides |
1.14.20.5 | PFNS-1 | - |
Populus deltoides |
2.1.1.B74 | flavone 7-O-methyltransferase | - |
Populus deltoides |
2.1.1.B74 | POMT-7 | - |
Populus deltoides |
2.1.1.B76 | POMT-7 | - |
Populus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.B74 | 28 | - |
assay at | Populus deltoides |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.20.5 | 6 | - |
optimum for the combined action of both enzymes, next optimal pH is 8.0, then pH 7.0 | Populus deltoides |
2.1.1.B74 | 6 | - |
recombinant enzyme | Populus deltoides |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.B74 | S-adenosyl-L-methionine | - |
Populus deltoides |