Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fu, X.T.; Pan, C.H.; Lin, H.; Kim, S.M.
    Gene cloning, expression, and characterization of a beta-agarase, AgaB34, from Agarivorans albus YKW-34 (2009), J. Microbiol. Biotechnol., 19, 257-264.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.81 additional information no activation of rAgaB34 by chelators (EDTA and EGTA), reducing reagents (beta-mercaptoethanol and DTT), and detergents (SDS and urea) Agarivorans albus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.81 DNA fragments (3.0-8.0 kb) ligated into vector pUC18 and transformed into Escherichia coli DH5alpha. Vector pUSH1, which produces a derivative of rAgaB34 with a 6 × His tag at its C-terminal, transformed into Escherichia coli DH5alpha Agarivorans albus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.81 Al3+
-
 Agarivorans albus
3.2.1.81 Cu2+
-
 Agarivorans albus
3.2.1.81 Mn2+
-
 Agarivorans albus
3.2.1.81 additional information no inhibition of rAgaB34 by metal ions Na+, K+, Mg2+, Ca2+, and Ba2+, chelators (EDTA and EGTA), reducing reagents (beta-mercaptoethanol and DTT), and detergents (SDS and urea) Agarivorans albus
3.2.1.81 Zn2+
-
 Agarivorans albus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.81 0.024
-
 agarose
-
 Agarivorans albus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.81 additional information no activation of rAgaB34 by Na+, K+, Mg2+, Ca2+, and Ba2+ Agarivorans albus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.81 49000
-
 x * 49000, SDS-PAGE Agarivorans albus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.81 Agarivorans albus A8W969
-
-
3.2.1.81 Agarivorans albus YKW-34 A8W969
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.81 rAgaB34 purified by ultrafiltration and on Ni+ column, 15fold with a yield of 80% Agarivorans albus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.81 16.9
-
 culture fluid Agarivorans albus
3.2.1.81 242.2
-
 15fold purified rAgaB34, with agarose as substrate at 40°C Agarivorans albus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.81 agarose + H2O
-
 Agarivorans albus neoagarohexaose + neoagarotetraose
-
 ?
3.2.1.81 agarose + H2O
-
 Agarivorans albus YKW-34 neoagarohexaose + neoagarotetraose
-
 ?
3.2.1.81 additional information neoagarotetraose can not be further hydrolyzed into neoagarobiose even after long incubation time Agarivorans albus ?
-
 ?
3.2.1.81 additional information neoagarotetraose can not be further hydrolyzed into neoagarobiose even after long incubation time Agarivorans albus YKW-34 ?
-
 ?
3.2.1.81 neoagarohexaose + H2O
-
 Agarivorans albus neoagarotetraose + neoagarobiose
-
 ?
3.2.1.81 neoagarohexaose + H2O
-
 Agarivorans albus YKW-34 neoagarotetraose + neoagarobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.81 ? x * 49000, SDS-PAGE Agarivorans albus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.81 AgaB34
-
 Agarivorans albus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.81 30
-
-
 Agarivorans albus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.81 50
-
-
 Agarivorans albus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.81 41
-
 agarose
-
 Agarivorans albus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.81 7
-
-
 Agarivorans albus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.81 5 9
-
 Agarivorans albus