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Literature summary extracted from
- R-stereoselective amidase from Rhodococcus erythropolis No. 7 acting on 4-chloro-3-hydroxybutyramide (2008), J. Microbiol. Biotechnol., 18, 552-559.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.4 | - |
Rhodococcus erythropolis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.4 | HgCl2 | - |
Rhodococcus erythropolis |  |
| 3.5.1.4 | iodoacetamide | - |
Rhodococcus erythropolis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.4 | 0.04 | - |
Benzamide | - |
Rhodococcus erythropolis | |
| 3.5.1.4 | 0.18 | - |
Isobutyramide | - |
Rhodococcus erythropolis | |
| 3.5.1.4 | 0.18 | - |
butyramide | - |
Rhodococcus erythropolis | |
| 3.5.1.4 | 1.3 | - |
Propionamide | - |
Rhodococcus erythropolis | |
| 3.5.1.4 | 4.79 | - |
Acrylamide | - |
Rhodococcus erythropolis | |
| 3.5.1.4 | 5.57 | - |
acetamide | - |
Rhodococcus erythropolis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.4 | Rhodococcus erythropolis | B4XEY3 | - |
- |
| 3.5.1.4 | Rhodococcus erythropolis No. 7 | B4XEY3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.4 | - |
Rhodococcus erythropolis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.4 | 4-chloro-3-hydroxybutyramide + H2O | - |
Rhodococcus erythropolis | 4-chloro-3-hydroxybutyric acid + NH3 | - |
? | |
| 3.5.1.4 | acetamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis | acetic acid + NH3 | - |
? | |
| 3.5.1.4 | acetamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis No. 7 | acetic acid + NH3 | - |
? | |
| 3.5.1.4 | acrylamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis | acrylic acid + NH3 | - |
? | |
| 3.5.1.4 | acrylamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis No. 7 | acrylic acid + NH3 | - |
? | |
| 3.5.1.4 | benzamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis | benzoic acid + NH3 | - |
? | |
| 3.5.1.4 | benzamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis No. 7 | benzoic acid + NH3 | - |
? | |
| 3.5.1.4 | butyramide + H2O | - |
Rhodococcus erythropolis | butyric acid + NH3 | - |
? | |
| 3.5.1.4 | isobutyramide + H2O | - |
Rhodococcus erythropolis | isobutyric acid + NH3 | - |
? | |
| 3.5.1.4 | additional information | enantioselectivity toward (R)-4-chloro-3-hydroxybutyramide: e.e value 52% (conversion yield 57%) | Rhodococcus erythropolis | ? | - |
? | |
| 3.5.1.4 | additional information | enantioselectivity toward (R)-4-chloro-3-hydroxybutyramide: e.e value 52% (conversion yield 57%) | Rhodococcus erythropolis No. 7 | ? | - |
? | |
| 3.5.1.4 | propionamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis | propionic acid + NH3 | - |
? | |
| 3.5.1.4 | propionamide + H2O | also acyl transfer activity in the presence of hydroxylamine | Rhodococcus erythropolis No. 7 | propionic acid + NH3 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.4 | amidase | - |
Rhodococcus erythropolis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 55 | - |
substrate: isobutyramide, no activity in Tris-HCl buffer | Rhodococcus erythropolis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 50 | - |
up to 50°C, 30 min | Rhodococcus erythropolis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.4 | 0.21 | - |
acetamide | - |
Rhodococcus erythropolis | |
| 3.5.1.4 | 0.48 | - |
Benzamide | - |
Rhodococcus erythropolis | |
| 3.5.1.4 | 0.58 | - |
Acrylamide | - |
Rhodococcus erythropolis | |
| 3.5.1.4 | 2.04 | - |
butyramide | - |
Rhodococcus erythropolis | |
| 3.5.1.4 | 4.03 | - |
Propionamide | - |
Rhodococcus erythropolis | |
| 3.5.1.4 | 5.68 | - |
Isobutyramide | - |
Rhodococcus erythropolis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 8.5 | - |
substrate: isobutyramide, no activity in Tris-HCl buffer | Rhodococcus erythropolis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 7 | 12 | 30 min | Rhodococcus erythropolis |
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