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Literature summary extracted from

  • Kiyohara, H.; Nagao, K.; Yano, K.
    Isolation and some properties of NAD-linked 2-carboxybenzaldehyde dehydrogenase in Alcaligenes faecalis AFK 2 grown on phenanthrene (1981), J. Gen. Appl. Microbiol., 27, 443-455.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.78 2-naphthoaldehyde 0.2 mM, 34.6% inhibition Alcaligenes faecalis
1.2.1.78 4-carboxybenzaldehyde 0.2 mM, 38.5% inhibition Alcaligenes faecalis
1.2.1.78 acetaldehyde 0.2 mM, 34.6% inhibition Alcaligenes faecalis
1.2.1.78 Ag+ 1 mM, complete inhibition Alcaligenes faecalis
1.2.1.78 Co2+ 1 mM, 40.2% inhibition Alcaligenes faecalis
1.2.1.78 Cu2+ 1 mM, complete inhibition Alcaligenes faecalis
1.2.1.78 Hg2+ 1 mM, 17.5% inhibition Alcaligenes faecalis
1.2.1.78 iso-phthalaldehyde 0.2 mM, 23.8% inhibition Alcaligenes faecalis
1.2.1.78 o-nitrobenzaldehyde 0.2 mM, 58.1% inhibition Alcaligenes faecalis
1.2.1.78 o-phthalaldehyde 0.2 mM, 91.4% inhibition Alcaligenes faecalis
1.2.1.78 p-chloromercuribenzoate 1 mM, complete inhibition Alcaligenes faecalis
1.2.1.78 p-dimethylamino benzaldehyde 0.2 mM, 32.7% inhibition Alcaligenes faecalis
1.2.1.78 propionaldehyde 0.2 mM, 31.7% inhibition Alcaligenes faecalis
1.2.1.78 Protocatechualdehyde 0.2 mM, 23.1% inhibition Alcaligenes faecalis
1.2.1.78 Salicylaldehyde 0.2 mM, 23.1% inhibition Alcaligenes faecalis
1.2.1.78 SDS 1 mM, 44.3% inhibition Alcaligenes faecalis
1.2.1.78 vertraldehyde 0.2 mM, 13.5% inhibition Alcaligenes faecalis
1.2.1.78 Zn2+ 1 mM, 71.7% inhibition Alcaligenes faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.78 0.054
-
 2-carboxybenzaldehyde pH 8.0, 30°C Alcaligenes faecalis
1.2.1.78 0.14
-
 NAD+ pH 8.0, 30°C Alcaligenes faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.78 41000
-
 4 * 41000, SDS-PAGE Alcaligenes faecalis
1.2.1.78 158000
-
 gel filtration Alcaligenes faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.78 2-carboxybenzaldehyde + NAD+ + H2O Alcaligenes faecalis the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene o-phthalic acid + NADH + H+
-
 ?
1.2.1.78 2-carboxybenzaldehyde + NAD+ + H2O Alcaligenes faecalis AFK2 the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene o-phthalic acid + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.78 Alcaligenes faecalis
-
-
-
1.2.1.78 Alcaligenes faecalis AFK2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.78
-
 Alcaligenes faecalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.78 culture condition:phenanthrene-grown cell
-
 Alcaligenes faecalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.78 130.6
-
-
 Alcaligenes faecalis

Storage Stability

EC Number Storage Stability Organism
1.2.1.78 4°C, activity in phosphate buffer is labile, 1 mM DTT stabilizes Alcaligenes faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.78 2-carboxybenzaldehyde + NAD+ + H2O the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene Alcaligenes faecalis o-phthalic acid + NADH + H+
-
 ?
1.2.1.78 2-carboxybenzaldehyde + NAD+ + H2O high specificity for 2-carboxybenzaldehyde as its substrate and for NAD+ as its electron acceptor Alcaligenes faecalis o-phthalic acid + NADH + H+
-
 ?
1.2.1.78 2-carboxybenzaldehyde + NAD+ + H2O the enzyme is involved in phenanthrene degradation, enzyme is inducible, increase in enzyme level by growth on phenanthrene Alcaligenes faecalis AFK2 o-phthalic acid + NADH + H+
-
 ?
1.2.1.78 2-carboxybenzaldehyde + NAD+ + H2O high specificity for 2-carboxybenzaldehyde as its substrate and for NAD+ as its electron acceptor Alcaligenes faecalis AFK2 o-phthalic acid + NADH + H+
-
 ?
1.2.1.78 additional information no activity with benzaldehyde, salicylaldehyde, iso-phthalaldehyde, 4-carboxybenzaldehyde and propionaldehyde Alcaligenes faecalis ?
-
 ?
1.2.1.78 additional information no activity with benzaldehyde, salicylaldehyde, iso-phthalaldehyde, 4-carboxybenzaldehyde and propionaldehyde Alcaligenes faecalis AFK2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.78 tetramer 4 * 41000, SDS-PAGE Alcaligenes faecalis

Synonyms

EC Number Synonyms Comment Organism
1.2.1.78 2CBAL dehydrogenase
-
 Alcaligenes faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.78 30
-
-
 Alcaligenes faecalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.78 30
-
 10 min, no loss of activity below 30°C Alcaligenes faecalis
1.2.1.78 40
-
 10 min, 25% loss of activity Alcaligenes faecalis
1.2.1.78 50
-
 10 min, 94% loss of activity Alcaligenes faecalis
1.2.1.78 60
-
 10 min, complete loss of activity Alcaligenes faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.78 8
-
-
 Alcaligenes faecalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.78 7 8.6 pH 7.0: about 60% of maximal activity, pH 8.6: about 70% of maximal activity Alcaligenes faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.78 NAD+ high specificity for NAD+ as electron acceptor Alcaligenes faecalis