BRENDA - Enzyme Database

Effects of some antibiotics on human erythrocyte glutathione reductase: an in vitro study

Senturk, M.; Kufrevioglu, O.I.; Ciftci, M.; J. Enzyme Inhib. Med. Chem. 23, 144-148 (2008)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.1.7
amikacin
-
Homo sapiens
1.8.1.7
amoxicillin
-
Homo sapiens
1.8.1.7
clindamycin
-
Homo sapiens
1.8.1.7
Lincomycin
-
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.7
ceftazidime
competitive inhibition
Homo sapiens
1.8.1.7
ceftriaxone
competitive inhibition
Homo sapiens
1.8.1.7
cefuroxime
competitive inhibition
Homo sapiens
1.8.1.7
Chloramphenicol
competitive inhibition
Homo sapiens
1.8.1.7
Imipenem
competitive inhibition
Homo sapiens
1.8.1.7
ornidazole
competitive inhibition
Homo sapiens
1.8.1.7
rifamycin
competitive inhibition
Homo sapiens
1.8.1.7
sulfanylacetamide
competitive inhibition
Homo sapiens
1.8.1.7
Vancomycin
noncompetitive inhibition
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.1.7
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.1.7
ammonium sulfate precipitation, 2',5'-ADP Sepharose 4B column chromatography and Sephadex G-200 gel filtration
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.1.7
erythrocyte
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.1.7
0.01
-
hemolysate, at 25°C, pH 8.0
Homo sapiens
1.8.1.7
28
-
after 2800fold purification, at 25°C, pH 8.0
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.7
glutathione disulfide + NADPH + H+
-
699224
Homo sapiens
glutathione + NADP+
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.7
FAD
-
Homo sapiens
1.8.1.7
NADPH
-
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.8.1.7
0.06
-
Imipenem
at 25°C, pH 8.0
Homo sapiens
1.8.1.7
0.085
-
sulfanylacetamide
at 25°C, pH 8.0
Homo sapiens
1.8.1.7
0.275
-
rifamycin
at 25°C, pH 8.0
Homo sapiens
1.8.1.7
3.59
-
ceftazidime
at 25°C, pH 8.0
Homo sapiens
1.8.1.7
3.71
-
ceftriaxone
at 25°C, pH 8.0
Homo sapiens
1.8.1.7
3.85
-
Chloramphenicol
at 25°C, pH 8.0
Homo sapiens
1.8.1.7
15.11
-
Vancomycin
at 25°C, pH 8.0
Homo sapiens
1.8.1.7
23.5
-
cefuroxime
at 25°C, pH 8.0
Homo sapiens
1.8.1.7
28.49
-
ornidazole
at 25°C, pH 8.0
Homo sapiens
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
1.8.1.7
0.03
-
at 25°C, pH 8.0
Homo sapiens
Imipenem
1.8.1.7
0.146
-
at 25°C, pH 8.0
Homo sapiens
rifamycin
1.8.1.7
0.59
-
at 25°C, pH 8.0
Homo sapiens
sulfanylacetamide
1.8.1.7
2.36
-
at 25°C, pH 8.0
Homo sapiens
Chloramphenicol
1.8.1.7
2.476
-
at 25°C, pH 8.0
Homo sapiens
ceftazidime
1.8.1.7
2.88
-
at 25°C, pH 8.0
Homo sapiens
ceftriaxone
1.8.1.7
4.83
-
at 25°C, pH 8.0
Homo sapiens
Vancomycin
1.8.1.7
15.43
-
at 25°C, pH 8.0
Homo sapiens
cefuroxime
1.8.1.7
19.63
-
at 25°C, pH 8.0
Homo sapiens
ornidazole
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.8.1.7
amikacin
-
Homo sapiens
1.8.1.7
amoxicillin
-
Homo sapiens
1.8.1.7
clindamycin
-
Homo sapiens
1.8.1.7
Lincomycin
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.1.7
FAD
-
Homo sapiens
1.8.1.7
NADPH
-
Homo sapiens
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
1.8.1.7
0.03
-
at 25°C, pH 8.0
Homo sapiens
Imipenem
1.8.1.7
0.146
-
at 25°C, pH 8.0
Homo sapiens
rifamycin
1.8.1.7
0.59
-
at 25°C, pH 8.0
Homo sapiens
sulfanylacetamide
1.8.1.7
2.36
-
at 25°C, pH 8.0
Homo sapiens
Chloramphenicol
1.8.1.7
2.476
-
at 25°C, pH 8.0
Homo sapiens
ceftazidime
1.8.1.7
2.88
-
at 25°C, pH 8.0
Homo sapiens
ceftriaxone
1.8.1.7
4.83
-
at 25°C, pH 8.0
Homo sapiens
Vancomycin
1.8.1.7
15.43
-
at 25°C, pH 8.0
Homo sapiens
cefuroxime
1.8.1.7
19.63
-
at 25°C, pH 8.0
Homo sapiens
ornidazole
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.8.1.7
ceftazidime
competitive inhibition
Homo sapiens
1.8.1.7
ceftriaxone
competitive inhibition
Homo sapiens
1.8.1.7
cefuroxime
competitive inhibition
Homo sapiens
1.8.1.7
Chloramphenicol
competitive inhibition
Homo sapiens
1.8.1.7
Imipenem
competitive inhibition
Homo sapiens
1.8.1.7
ornidazole
competitive inhibition
Homo sapiens
1.8.1.7
rifamycin
competitive inhibition
Homo sapiens
1.8.1.7
sulfanylacetamide
competitive inhibition
Homo sapiens
1.8.1.7
Vancomycin
noncompetitive inhibition
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.8.1.7
0.06
-
Imipenem
at 25°C, pH 8.0
Homo sapiens
1.8.1.7
0.085
-
sulfanylacetamide
at 25°C, pH 8.0
Homo sapiens
1.8.1.7
0.275
-
rifamycin
at 25°C, pH 8.0
Homo sapiens
1.8.1.7
3.59
-
ceftazidime
at 25°C, pH 8.0
Homo sapiens
1.8.1.7
3.71
-
ceftriaxone
at 25°C, pH 8.0
Homo sapiens
1.8.1.7
3.85
-
Chloramphenicol
at 25°C, pH 8.0
Homo sapiens
1.8.1.7
15.11
-
Vancomycin
at 25°C, pH 8.0
Homo sapiens
1.8.1.7
23.5
-
cefuroxime
at 25°C, pH 8.0
Homo sapiens
1.8.1.7
28.49
-
ornidazole
at 25°C, pH 8.0
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.1.7
ammonium sulfate precipitation, 2',5'-ADP Sepharose 4B column chromatography and Sephadex G-200 gel filtration
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.1.7
erythrocyte
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.8.1.7
0.01
-
hemolysate, at 25°C, pH 8.0
Homo sapiens
1.8.1.7
28
-
after 2800fold purification, at 25°C, pH 8.0
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.1.7
glutathione disulfide + NADPH + H+
-
699224
Homo sapiens
glutathione + NADP+
-
-
-
?