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Literature summary extracted from

  • Yang, S.; Lin, Q.; Lu, Z.; Lu, F.; Bie, X.; Zou, X.; Sun, L.
    Characterization of a novel glutamate decarboxylase from Streptococcus salivarius ssp. thermophilus Y2 (2008), J. Chem. Technol. Biotechnol., 83, 855-861.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.15 gene gadB, DNA and amino acid sequence determination and analysis Streptococcus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.15 AgNO3 complete inhibition at 5mM Streptococcus thermophilus
4.1.1.15 CoCl2 62% inhibition at 5mM Streptococcus thermophilus
4.1.1.15 CuSO4 93% inhibition at 5mM Streptococcus thermophilus
4.1.1.15 FeSO4 92% inhibition at 5mM Streptococcus thermophilus
4.1.1.15 MnSO4 88% inhibition at 5mM Streptococcus thermophilus
4.1.1.15 additional information poor inhibition by LiCl, CaCl2, and KCl. MgCl2 and NaCl have no effect on enzyme activity Streptococcus thermophilus
4.1.1.15 Na2SO4 10% inhibition at 5mM Streptococcus thermophilus
4.1.1.15 ZnSO4 80.4% inhibition at 5mM Streptococcus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.15 2.3
-
 L-glutamate pH 4.0, 40°C Streptococcus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.15 BaCl2 activates by 22% at 5 mM Streptococcus thermophilus
4.1.1.15 additional information MgCl2 and NaCl have no effect on enzyme activity Streptococcus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.15 46900
-
 2 * 46900, SDS-PAGE Streptococcus thermophilus
4.1.1.15 103600
-
 gel filtration Streptococcus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.15 L-glutamate Streptococcus thermophilus
-
 4-aminobutanoate + CO2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.15 Streptococcus thermophilus Q0GE18 ssp. thermophilus Y2, gene gadB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.15 native enzyme 22fold to homogeneity by ammonium sulfate fractionation, precipitation in N-methylpiperazine, anion exchange and hydrophobic interaction chromatography, and gel filtration Streptococcus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.15 103.5
-
 purified enzyme Streptococcus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.15 L-glutamate
-
 Streptococcus thermophilus 4-aminobutanoate + CO2
-
 ir
4.1.1.15 L-glutamate irreversible alpha-decarboxylation of L-glutamate, the enzyme is highly specific for L-glutamate, no activity with D-glutamate and 23 other amino acids, overview Streptococcus thermophilus 4-aminobutanoate + CO2
-
 ir

Subunits

EC Number Subunits Comment Organism
4.1.1.15 dimer 2 * 46900, SDS-PAGE Streptococcus thermophilus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.15 GAD
-
 Streptococcus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.15 55
-
-
 Streptococcus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.15 40 80 about 40% of maximal activity at 40°C and 80°C Streptococcus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.15 40
-
 purified enzyme, pH 4.0, loss of 10% activity after 5 h, stable below Streptococcus thermophilus
4.1.1.15 50
-
 purified enzyme, pH 4.0, loss of 3% activity after 1 h Streptococcus thermophilus
4.1.1.15 60
-
 purified enzyme, pH 4.0, loss of about 15% activity after 5 h Streptococcus thermophilus
4.1.1.15 80
-
 purified enzyme, pH 4.0, loss of about 50% activity after 5 h Streptococcus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.15 4
-
-
 Streptococcus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.15 3.5 5.2 pH 3.5: about 40% of maximal activity, pH 4.5: about 65% of maximal activity, pH 5.0: about 50% of maximal activity pH 5.2: about 40% of maximal activity Streptococcus thermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.15 3.5
-
 purified enzyme, loss of about 35% activity at 40°C after 5 h Streptococcus thermophilus
4.1.1.15 4
-
 purified enzyme, 40°C, loss of 3% activity after 1 h, and of 10% activity after 5 h Streptococcus thermophilus
4.1.1.15 4
-
 purified enzyme, maximally stable at pH 4.0 Streptococcus thermophilus
4.1.1.15 5.5
-
 purified enzyme, loss of about 40% activity at 40°C after 5 h Streptococcus thermophilus