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  • Pisanelli, I.; Kujawa, M.; Spadiut, O.; Kittl, R.; Halada, P.; Volc, J.; Mozuch, M.D.; Kersten, P.; Haltrich, D.; Peterbauer, C.
    Pyranose 2-oxidase from Phanerochaete chrysosporium--expression in E. coli and biochemical characterization (2009), J. Biotechnol., 142, 97-106.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.10 heterologously expressed in Escherichia coli DH5 alpha as His-tagged protein Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.10 0.042
-
 1,4-benzoquinone substrate 1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 4.5 Phanerodontia chrysosporium
1.1.3.10 0.051
-
 2,6-dichloroindophenol substrate 2,6-dichloroindophenol (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 0.09
-
 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical substrate 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 0.11
-
 1,4-benzoquinone substrate 1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 0.21
-
 2-methoxy-1,4-benzoquinone substrate 2-methoxy-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 0.29
-
 ferricenium hexafluorophosphate substrate ferricenium hexafluorophosphate (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 8.0 Phanerodontia chrysosporium
1.1.3.10 0.31
-
 2-chloro-1,4-benzoquinone substrate 2-chloro-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 0.33
-
 ferricenium hexafluorophosphate substrate ferricenium hexafluorophosphate (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 0.51
-
 2-methyl-1,4-benzoquinone substrate 2-methyl-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 0.64
-
 tetrafluoro-1,4-benzoquinone substrate tetrafluoro-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 0.84
-
 D-glucose substrate D-glucose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 1.22
-
 O2 substrate O2 (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 1.59
-
 2,6-dimethyl-1,4-benzoquinone substrate 2,6-dimethyl-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 2.94
-
 D-galactose substrate D-galactose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 3.43
-
 ferricyanide substrate ferricyanide (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 20.9
-
 D-xylose substrate D-xylose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 23.5
-
 L-sorbose substrate L-sorbose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 103
-
 D-fructose substrate D-fructose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.10 65000
-
 one subunit of recombinant expressed protein Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Phanerodontia chrysosporium Q6QWR1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.10 homogenized in a French press, one-step immobilized metal affinity chromatography, concentrated in 50mM potassium phosphate buffer Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?
1.1.3.10 2,6-dichloroindophenol + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?
1.1.3.10 2,6-dimethyl-1,4-benzoquinone + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?
1.1.3.10 2-chloro-1,4-benzoquinone + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?
1.1.3.10 2-methoxy-1,4-benzoquinone + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?
1.1.3.10 D-fructose + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?
1.1.3.10 D-galactose + 1,4-benzoquinone
-
 Phanerodontia chrysosporium 2-dehydro-D-galactose + 1,4-hydroquinone
-
 ?
1.1.3.10 D-galactose + O2
-
 Phanerodontia chrysosporium 2-dehydro-D-galactose + H2O2
-
 ?
1.1.3.10 D-glucose + 1,4-benzoquinone
-
 Phanerodontia chrysosporium 2-dehydro-D-glucose + 1,4-hydroquinone
-
 ?
1.1.3.10 D-glucose + 2-methyl-1,4-benzoquinone
-
 Phanerodontia chrysosporium 2-dehydro-D-glucose + 2-methylhydroquinone
-
 r
1.1.3.10 D-glucose + ferricyanide
-
 Phanerodontia chrysosporium 2-dehydro-D-glucose + ferrocyanide
-
 r
1.1.3.10 D-glucose + O2
-
 Phanerodontia chrysosporium 2-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 D-xylose + O2
-
 Phanerodontia chrysosporium D-xylosone + H2O2
-
 ?
1.1.3.10 ferricenium hexafluorophosphate + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?
1.1.3.10 L-sorbose + O2
-
 Phanerodontia chrysosporium 5-dehydro-D-fructose + H2O2
-
 ?
1.1.3.10 tetrafluoro-1,4-benzoquinone + O2
-
 Phanerodontia chrysosporium ? + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.10 homotetramer suggested by observed molecular weight Phanerodontia chrysosporium

Synonyms

EC Number Synonyms Comment Organism
1.1.3.10 P2Ox
-
 Phanerodontia chrysosporium
1.1.3.10 pyranose 2-Oxidase
-
 Phanerodontia chrysosporium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.10 0.12
-
 2-methyl-1,4-benzoquinone substrate 2-methyl-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 0.15
-
 2-chloro-1,4-benzoquinone substrate 2-chloro-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 0.3
-
 ferricyanide substrate ferricyanide (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 1.33
-
 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical substrate 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) cation radical (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 1.88
-
 D-fructose substrate D-fructose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 4.87
-
 D-galactose substrate D-galactose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 6.61
-
 tetrafluoro-1,4-benzoquinone substrate tetrafluoro-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 8.69
-
 2,6-dimethyl-1,4-benzoquinone substrate 2,6-dimethyl-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 40.1
-
 2-methoxy-1,4-benzoquinone substrate 2-methoxy-1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 44.9
-
 D-xylose substrate D-xylose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 58.8
-
 L-sorbose substrate L-sorbose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 83.1
-
 D-glucose substrate D-glucose (constant O2 concentration), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 108
-
 2,6-dichloroindophenol substrate 2,6-dichloroindophenol (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 109
-
 O2 substrate O2 (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 228
-
 ferricenium hexafluorophosphate substrate ferricenium hexafluorophosphate (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 400
-
 1,4-benzoquinone substrate 1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 6.5 Phanerodontia chrysosporium
1.1.3.10 477
-
 1,4-benzoquinone substrate 1,4-benzoquinone (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 4.5 Phanerodontia chrysosporium
1.1.3.10 549
-
 ferricenium hexafluorophosphate substrate ferricenium hexafluorophosphate (constant D-glucose concentration, 20 mM), activity determined spectrophotometrically at 420 nm by measuring formation of H2O2 with a horse-radish peroxidase-coupled assay using 2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid) as the chromogen, 30°C, pH 8.0 Phanerodontia chrysosporium