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Literature summary extracted from
- Site-directed mutagenesis of manganese peroxidase from Phanerochaete chrysosporium in an in vitro expression system (2009), J. Biotechnol., 139, 176-178.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.11.1.13 | degradation | Mn peroxidases are of much interest biotechnologically because of their potentially applications in bioremdeial waste treatment and in catalyzing difficult chemical transfromations | Phanerodontia chrysosporium |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.11.1.13 | into the vector pET-22b, used for in vitro transcription and translation in a wheat germ extract system | Phanerodontia chrysosporium |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.11.1.13 | N131D | mutant displays a similar catalysis pattern to that of wild-type enzyme, Asn131 is the only potential glycosylation site | Phanerodontia chrysosporium |
| 1.11.1.13 | R42A | mutant displays a similar catalysis pattern to that of wild-type enzyme, Arg42 is forming the peroxide binding pocket | Phanerodontia chrysosporium |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.11.1.13 | extracellular | - |
Phanerodontia chrysosporium | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.13 | Manganese | - |
Phanerodontia chrysosporium |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 43000 | - |
determined by Western blot analysis | Phanerodontia chrysosporium |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.13 | Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | - |
Mn3+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.13 | Phanerodontia chrysosporium | Q02567 | - |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | additional information | - |
0.055 U/ml, R42A mutant, in the presence of 2,6-dimethoxyphenol | Phanerodontia chrysosporium |
| 1.11.1.13 | additional information | - |
0.069 U/ml, N131D mutant, in the presence of 2,6-dimethoxyphenol | Phanerodontia chrysosporium |
| 1.11.1.13 | additional information | - |
0.076 U/ml, wild-type enzyme, in the presence of 2,6-dimethoxyphenol | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.13 | Mn2+ + 2,6-dimethoxyphenol + H2O2 | - |
Phanerodontia chrysosporium | ? | - |
? | |
| 1.11.1.13 | Mn2+ + H+ + H2O2 | - |
Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.11.1.13 | manganese peroxidase | - |
Phanerodontia chrysosporium |
| 1.11.1.13 | MnP | - |
Phanerodontia chrysosporium |
| 1.11.1.13 | mnp1 | - |
Phanerodontia chrysosporium |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.13 | heme | - |
Phanerodontia chrysosporium | |
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