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Literature summary extracted from

  • Cheng, M.; Takenaka, S.; Aoki, S.; Murakami, S.; Aoki, K.
    Purification and characterization of an eggshell membrane decomposing protease from Pseudomonas aeruginosa strain ME-4 (2009), J. Biosci. Bioeng., 107, 373-378.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.26 EDTA 1 mM, 28% residual activity Pseudomonas aeruginosa
3.4.24.26 o-phenanthroline 1 mM, 16% residual activity Pseudomonas aeruginosa
3.4.24.26 phosphoramidon 1 mM, 10% residual activity Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.26 extracellular
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.26 33000
-
 x * 33000, SDS-PAGE Pseudomonas aeruginosa

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.24.26 2-propanol 25% v/v, 36% residual activity Pseudomonas aeruginosa
3.4.24.26 Acetone 25% v/v, 31% residual activity Pseudomonas aeruginosa
3.4.24.26 dimethyl sulfoxide 25% v/v, 11% residual activity Pseudomonas aeruginosa
3.4.24.26 Ethanol 25% v/v, 24% residual activity Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.26 Pseudomonas aeruginosa
-
-
-
3.4.24.26 Pseudomonas aeruginosa ME-4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.26
-
 Pseudomonas aeruginosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.26 6.4
-
 pH 8.0, 30°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.26 Bovine serum albumin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 Bovine serum albumin + H2O
-
 Pseudomonas aeruginosa ME-4 ?
-
 ?
3.4.24.26 casein + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 casein + H2O
-
 Pseudomonas aeruginosa ME-4 ?
-
 ?
3.4.24.26 Collagen + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 Collagen + H2O
-
 Pseudomonas aeruginosa ME-4 ?
-
 ?
3.4.24.26 eggshell membrane + H2O
-
 Pseudomonas aeruginosa Val-Leu-Pro-Pro + (X)-Val-Pro-Pro + Trp + ?
-
 ?
3.4.24.26 elastin-orcin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 Gelatin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 additional information enzyme prefers Ser at position P1, Lys at P2 and hydropobic amino acids at the P1' and P2' positions Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 additional information no substrate: N-succinyl-Ala-Ala-Ala-p-nitroanilide, N-succinyl-Ala-Pro-Ala-p-nitroanilide Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 additional information enzyme prefers Ser at position P1, Lys at P2 and hydropobic amino acids at the P1' and P2' positions Pseudomonas aeruginosa ME-4 ?
-
 ?
3.4.24.26 additional information no substrate: N-succinyl-Ala-Ala-Ala-p-nitroanilide, N-succinyl-Ala-Pro-Ala-p-nitroanilide Pseudomonas aeruginosa ME-4 ?
-
 ?
3.4.24.26 ovalbumin + H2O
-
 Pseudomonas aeruginosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.26 ? x * 33000, SDS-PAGE Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.26 50
-
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.26 30 60
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.26 8
-
-
 Pseudomonas aeruginosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.26 6 9.5
-
 Pseudomonas aeruginosa