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Literature summary extracted from

  • Kim, J.H.; Sunako, M.; Ono, H.; Murooka, Y.; Fukusaki, E.; Yamashita, M.
    Characterization of the C-terminal truncated form of amylopullulanase from Lactobacillus plantarum L137 (2009), J. Biosci. Bioeng., 107, 124-129.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.135 NCL21 with plasmid pRN-PulSa, pRN-Puldelta3 Lactiplantibacillus plantarum

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.135 additional information C-terminal truncated ApuA produced with Escherichia coli plasmid, and transformed into Lactobacillus plantarum Lactiplantibacillus plantarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.135 alpha-cyclodextrin moderate inhibition by 10 mM, 30°C, 1 h Lactiplantibacillus plantarum
3.2.1.135 gamma-cyclodextrin moderate inhibition by 10 mM, 30°C, 1 h Lactiplantibacillus plantarum
3.2.1.135 Guanidine-HCl inhibition of both enzyme variants at 1 M, 30°C, 1 h Lactiplantibacillus plantarum
3.2.1.135 additional information no inhibition by 10 mM EDTA and beta-cyclodextrin, 30°C, 1 h Lactiplantibacillus plantarum
3.2.1.135 N-bromosuccinimide strong inhibition by 0.1 mM, 30°C, 1 h Lactiplantibacillus plantarum
3.2.1.135 Urea inhibition of both enzyme variants at 5 M, 30°C, 1 h Lactiplantibacillus plantarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.135 additional information
-
 additional information 2.6 g/l, amylose, full length enzyme, pH 4.5, 40°C Lactiplantibacillus plantarum
3.2.1.135 additional information
-
 additional information 2.6 g/l, pullulan, C-terminal truncated enzyme ApuADELTA, pH 4.5, 40°C Lactiplantibacillus plantarum
3.2.1.135 additional information
-
 additional information 3.3 g/l, amylose, C-terminal truncated enzyme ApuADELTA, pH 4.5, 40°C Lactiplantibacillus plantarum
3.2.1.135 additional information
-
 additional information 5.0 g/l, starch, C-terminal truncated enzyme ApuADELTA, pH 4.5, 40°C Lactiplantibacillus plantarum
3.2.1.135 additional information
-
 additional information 6.9 g/l, pullulan, full length enzyme, pH 4.5, 40°C Lactiplantibacillus plantarum
3.2.1.135 additional information
-
 additional information 7.7 g/l, starch, full length enzyme, pH 4.5, 40°C Lactiplantibacillus plantarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.135 CaCl2 1 mM C-terminal truncated enzyme ApuADELTA: amylase activity: 105%, pullulanase activity: 94% Lactiplantibacillus plantarum
3.2.1.135 CaCl2 1 mM full length enzyme: amylase activity: 111%, pullulanase activity: 101% Lactiplantibacillus plantarum
3.2.1.135 CoCl2 1 mM C-terminal truncated enzyme ApuADELTA: amylase activity: 128%, pullulanase activity: 130% Lactiplantibacillus plantarum
3.2.1.135 CoCl2 1 mM full length enzyme: amylase activity: 150%, pullulanase activity: 140% Lactiplantibacillus plantarum
3.2.1.135 KCl 50 mM C-terminal truncated enzyme ApuADELTA: amylase activity: 106%, pullulanase activity: 93% Lactiplantibacillus plantarum
3.2.1.135 KCl 50 mM full length enzyme: amylase activity: 112%, pullulanase activity: 99% Lactiplantibacillus plantarum
3.2.1.135 MnCl2 1 mM C-terminal truncated enzyme ApuADELTA: amylase activity: 109%, pullulanase activity: 121% Lactiplantibacillus plantarum
3.2.1.135 MnCl2 1 mM full length enzyme: amylase activity: 133%, pullulanase activity: 133% Lactiplantibacillus plantarum
3.2.1.135 NaCl 50 mM C-terminal truncated enzyme ApuADELTA: amylase activity: 103%, pullulanase activity: 94% Lactiplantibacillus plantarum
3.2.1.135 NaCl 50 mM full length enzyme: amylase activity: 107%, pullulanase activity: 95% Lactiplantibacillus plantarum
3.2.1.135 NiCl2 1 mM C-terminal truncated enzyme ApuADELTA: amylase activity: 87%, pullulanase activity: 100% Lactiplantibacillus plantarum
3.2.1.135 NiCl2 1 mM full length enzyme: amylase activity: 116%, pullulanase activity: 112% Lactiplantibacillus plantarum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.135 Lactiplantibacillus plantarum
-
 L137
-
3.2.1.135 Lactiplantibacillus plantarum ATCC BAA-793
-
 L137
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.135 supernatant ultrafiltrated, DEAE Sepharose CLl-4B, Superose 6 Lactiplantibacillus plantarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.135 0.00000431
-
 full length enzyme Lactiplantibacillus plantarum
3.2.1.135 0.00000596
-
 C-terminal truncated enzyme ApuADELTA Lactiplantibacillus plantarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.135 amylose + H2O 61% larger oligosaccharides than maltoocataose after 60 min of C-terminal truncated enzyme ApuADELTA activity compared to 92.6% of full length enzyme activity Lactiplantibacillus plantarum maltotriose + maltotetraose + maltopentaose no glucose, nor maltose ?
3.2.1.135 amylose + H2O 61% larger oligosaccharides than maltoocataose after 60 min of C-terminal truncated enzyme ApuADELTA activity compared to 92.6% of full length enzyme activity Lactiplantibacillus plantarum ATCC BAA-793 maltotriose + maltotetraose + maltopentaose no glucose, nor maltose ?
3.2.1.135 pullulan + H2O no larger oligosaccharides than maltoocataose after 5 min of C-terminal truncated enzyme ApuADELTA activity compared to 95.5% of ApuA activity Lactiplantibacillus plantarum maltotriose + maltopentaose + maltohexaose + maltoheptaose no glucose, nor maltose ?
3.2.1.135 pullulan + H2O no larger oligosaccharides than maltoocataose after 5 min of C-terminal truncated enzyme ApuADELTA activity compared to 95.5% of ApuA activity Lactiplantibacillus plantarum ATCC BAA-793 maltotriose + maltopentaose + maltohexaose + maltoheptaose no glucose, nor maltose ?
3.2.1.135 soluble starch + H2O
-
 Lactiplantibacillus plantarum maltotriose + maltotetraose no glucose, nor maltose ?
3.2.1.135 soluble starch + H2O
-
 Lactiplantibacillus plantarum ATCC BAA-793 maltotriose + maltotetraose no glucose, nor maltose ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.135 amylopullalanase
-
 Lactiplantibacillus plantarum
3.2.1.135 ApuA full length enzyme Lactiplantibacillus plantarum
3.2.1.135 ApuADELTA C-terminal truncated enzyme Lactiplantibacillus plantarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.135 40
-
 40°C and 45°C with about 100% activity for full length enzyme and C-terminal truncated enzyme ApuADELTA, pH 4, starch as substrate Lactiplantibacillus plantarum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.135 20 60 C-terminal truncated enzyme ApuADELTA with only slightly higher activities than full length enzyme, especially at higher than optimal temperatures, about 100% activity at 40-45°C, 60-90% activity at 20-35°C and 50°C, 20-40% activity at 55-60°C, 0.1 M sodium acetate butter, pH 4.5, starch as substrate Lactiplantibacillus plantarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.135 20 60 C-terminal truncated enzyme ApuADELTA, 100% activity at 20-30°C, 50% at 45°C, pre-incubation at given temperatures for 1 h, pH 4.5, residual activity measured at 40°C, starch as substrate Lactiplantibacillus plantarum
3.2.1.135 20 60 full length enzyme, 100% activity at 20-30°C, 50% activity at 41°C, pre-incubation at given temperatures for 1 h, pH 4.5, residual activity measured at 40°C, starch as substrate Lactiplantibacillus plantarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.135 4
-
 full length enzyme and C-terminal truncated enzyme ApuADELTA with optimum at pH 4-4.5, 0.1 M buffers pH 2.5-4: glycine-HCl, pH 3.5-6: acetate, pH 6-8: phosphate, substrate: soluble starch Lactiplantibacillus plantarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.135 2 7 40°C, 0.1 M buffers pH 2.5-4: glycine-HCl, pH 3.5-6: acetate, pH 6-8: phosphate, substrate: soluble starch Lactiplantibacillus plantarum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.135 2 8 C-terminal truncated enzyme ApuADELTA, pre-incubation for 1 h, 40°C, 0.1 M buffers pH 2.5-8, 90-100% activity from pH 2.5-6.6, about 80% activity at pH 7, about 60% at pH 7.5, about 30% activity at pH 8 Lactiplantibacillus plantarum
3.2.1.135 2 8 full length enzyme slightly less stable than C-terminal truncated enzyme ApuADELTA, pre-incubation for 1 h, 40°C, 0.1 M buffers pH 2.5-8, 90-100% activity from pH 2.5-6, about 80% activity at pH 6.5, about 50% at pH 7, between 20-30% activity at pH 7.5 and 8 Lactiplantibacillus plantarum