BRENDA - Enzyme Database

Kinetic and redox properties of MnP II, a major manganese peroxidase isoenzyme from Panus tigrinus CBS 577.79

Petruccioli, M.; Frasconi, M.; Quaratino, D.; Covino, S.; Favero, G.; Mazzei, F.; Federici, F.; DAnnibale, A.; J. Biol. Inorg. Chem. 14, 1153-1163 (2009)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.11.1.13
diphosphate
diphosphate proves to be the worst chelator, leading to a specific activity about 4.6% of that obtained with tartrate
Lentinus tigrinus
1.11.1.13
Lactate
lactate serves as a chelator for the Mn3+ chelate formation, leading to a specific activity about 48.4% of that obtained with tartrate
Lentinus tigrinus
1.11.1.13
malonate
the highest stimulation of 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate, malonate also serves as a chelator for the Mn3+ chelate formation, leading to a specific activity about 54.8% of that obtained with tartrate
Lentinus tigrinus
1.11.1.13
oxalate
oxalate serves as a chelator for the Mn3+ chelate formation, leading to a specific activity about 64.7% of that obtained with tartrate
Lentinus tigrinus
1.11.1.13
Tartrate
the highest rate of Mn3+ formation is obtained with 10 mM tartrate
Lentinus tigrinus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.13
0.016
-
H2O2
; pH 5.5, 45°C
Lentinus tigrinus
1.11.1.13
0.0713
-
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
Lentinus tigrinus
1.11.1.13
0.0713
-
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) Mn2+
-
Lentinus tigrinus
1.11.1.13
0.076
-
2,6-dimethoxyphenol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
0.124
-
Mn2+
; pH 5.5, 45°C
Lentinus tigrinus
1.11.1.13
0.196
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
Lentinus tigrinus
1.11.1.13
0.196
-
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
0.213
-
guaiacol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
0.293
-
pyrogallol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
0.56
-
veratryl alcohol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
2.3
-
guaiacol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
Lentinus tigrinus
1.11.1.13
3.74
-
pyrogallol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
Lentinus tigrinus
1.11.1.13
6.954
-
2,6-dimethoxyphenol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
Lentinus tigrinus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.11.1.13
Manganese
-
Lentinus tigrinus
1.11.1.13
Mn2+
veratryl alcohol oxidation requires the simultaneous presence of H2O2 and Mn2+
Lentinus tigrinus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.13
50500
-
determined by gel filtration and SDS-PAGE; gel filtration
Lentinus tigrinus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.11.1.13
Mn2+ + H+ + H2O2
Lentinus tigrinus
-
Mn3+ + H2O
-
-
?
1.11.1.13
Mn2+ + H+ + H2O2
Lentinus tigrinus CBS 577.79
-
Mn3+ + H2O
-
-
?
1.11.1.13
Mn2+ + H+ + H2O2
Lentinus tigrinus 577.79
-
Mn3+ + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.11.1.13
Lentinus tigrinus
-
-
-
1.11.1.13
Lentinus tigrinus 577.79
-
-
-
1.11.1.13
Lentinus tigrinus CBS 577.79
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
1.11.1.13
glycoprotein
N-glycosylation of about 5.3% of the high-mannose type; the enzyme displays an extent of N-glycosylation of about 5.3% of the high-mannose type
Lentinus tigrinus
Purification (Commentary)
EC Number
Commentary
Organism
1.11.1.13
purification scheme involves ultrafiltration, affinity chromatography on concanavalin-A Sepharose and gel filtration; ultrafiltration, Concanavalin-A Sepharose column chromatography and Superdex 75 gel filtration
Lentinus tigrinus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.11.1.13
40
-
culture supernatant, pH 5.5, 45°C; purification step culture supernatant
Lentinus tigrinus
1.11.1.13
41
-
purification step ultrafiltration
Lentinus tigrinus
1.11.1.13
169
-
purification step chromatography on a concanavalin-A Sepharose column
Lentinus tigrinus
1.11.1.13
288
-
after 7.2fold purification, pH 5.5, 45°C; purification step gel filtration
Lentinus tigrinus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.13
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + Mn2+ + ?
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + Mn2+ + ?
-
699016
Lentinus tigrinus CBS 577.79
?
-
-
-
?
1.11.1.13
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + Mn2+ + ?
-
699016
Lentinus tigrinus 577.79
?
-
-
-
?
1.11.1.13
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid + H2O2
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid + H2O2 + Mn2+
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
2,6-dimethoxyphenol + H2O2
the highest relative activity for 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
2,6-dimethoxyphenol + H2O2
the highest relative activity for 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate
699016
Lentinus tigrinus CBS 577.79
?
-
-
-
?
1.11.1.13
2,6-dimethoxyphenol + H2O2
the highest relative activity for 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate
699016
Lentinus tigrinus 577.79
?
-
-
-
?
1.11.1.13
2,6-dimethoxyphenol + H2O2 + Mn2+
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
2,6-dimethoxyphenol + Mn2+ + ?
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
guaiacol + H2O2
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
guaiacol + H2O2
-
699016
Lentinus tigrinus CBS 577.79
?
-
-
-
?
1.11.1.13
guaiacol + H2O2
-
699016
Lentinus tigrinus 577.79
?
-
-
-
?
1.11.1.13
guaiacol + H2O2 + Mn2+
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
guaiacol + Mn2+ + ?
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
Mn2+ + H+ + H2O2
-
699016
Lentinus tigrinus
Mn3+ + H2O
-
-
-
?
1.11.1.13
Mn2+ + H+ + H2O2
-
699016
Lentinus tigrinus CBS 577.79
Mn3+ + H2O
-
-
-
?
1.11.1.13
Mn2+ + H+ + H2O2
-
699016
Lentinus tigrinus 577.79
Mn3+ + H2O
-
-
-
?
1.11.1.13
pyrogallol + H2O2
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
pyrogallol + H2O2
-
699016
Lentinus tigrinus CBS 577.79
?
-
-
-
?
1.11.1.13
pyrogallol + H2O2
-
699016
Lentinus tigrinus 577.79
?
-
-
-
?
1.11.1.13
pyrogallol + H2O2 + Mn2+
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
pyrogallol + Mn2+ + ?
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
veratryl alcohol + H2O2 + Mn2+
veratryl alcohol oxidation requires the simultaneous presence of H2O2 and Mn2+
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
veratryl alcohol + Mn2+ + ?
-
699016
Lentinus tigrinus
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.11.1.13
monomer
; 1 * 50500, SDS-PAGE
Lentinus tigrinus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.11.1.13
45
-
; optimum temperature for the oxidation of Mn2+ and DMOP
Lentinus tigrinus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.11.1.13
10
60
the enzyme shows relative activities of 34, 50, and 47% at 10, 40, and 60°C, respectively
Lentinus tigrinus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.11.1.13
50
70
the enzyme is rather unstable at 50, 60, and 70°C, resulting in half-lives of 660, 105, and 41 s, respectively
Lentinus tigrinus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.11.1.13
0.45
-
veratryl alcohol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
0.74
-
guaiacol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
Lentinus tigrinus
1.11.1.13
1.87
-
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
Lentinus tigrinus
1.11.1.13
1.87
-
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) Mn2+
-
Lentinus tigrinus
1.11.1.13
5.67
-
2,6-dimethoxyphenol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
Lentinus tigrinus
1.11.1.13
8.92
-
guaiacol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
9.98
-
pyrogallol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
Lentinus tigrinus
1.11.1.13
20.2
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
Lentinus tigrinus
1.11.1.13
20.2
-
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
20.95
-
pyrogallol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
26.26
-
2,6-dimethoxyphenol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
121
-
H2O2
; with 1.5 mM Mn2+ as cosubstrate, pH 5.5, 45°C
Lentinus tigrinus
1.11.1.13
242.4
-
Mn2+
; with 0.2 mM H2O2 as cosubstrate, pH 5.5, 45°C
Lentinus tigrinus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.13
3
-
optimal pH for the Mn-dependent oxidation of veratryl alcohol; optimum pH for the Mn2+-dependent oxidation of veratryl alehyde
Lentinus tigrinus
1.11.1.13
3.5
4.5
pH optimum for Mn2+-independent DMOP oxidation
Lentinus tigrinus
1.11.1.13
4
4.5
pH optimum for Mn2+-dependent DMOP oxidation
Lentinus tigrinus
1.11.1.13
5.5
-
-
Lentinus tigrinus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.11.1.13
3.5
4.5
optima for Mn2+-independent 2,6-dimethoxyphenol oxidation
Lentinus tigrinus
1.11.1.13
3.5
6.5
Mn2+ oxidation shows an optimum in the range 5.5-5.75 and negligible activity at pH values below 3.5 and above 6.5
Lentinus tigrinus
1.11.1.13
4
4.5
optima for Mn2+-dependent 2,6-dimethoxyphenol oxidation
Lentinus tigrinus
1.11.1.13
5.5
5.8
the pH-activity profile for Mn2+ oxidation shows an optimum in the range 5.5 - 5.75 and negligible activity at pHs below 3.5 and above 6.5
Lentinus tigrinus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.11.1.13
3.5
5.5
the enzyme shows a half-life of 8.8 h at pH 3.5, the half-lives of MnP activity at pH 4.5, 5.0, and 5.5 are 89.5, 99, and 101 h, respectively
Lentinus tigrinus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.13
heme
the heme content is 0.96 mol per mole of the enzyme
Lentinus tigrinus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.11.1.13
Lentinus tigrinus
-
-
4.07
1.11.1.13
Lentinus tigrinus
isoelectric focusing
-
4.1
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.11.1.13
diphosphate
diphosphate proves to be the worst chelator, leading to a specific activity about 4.6% of that obtained with tartrate
Lentinus tigrinus
1.11.1.13
Lactate
lactate serves as a chelator for the Mn3+ chelate formation, leading to a specific activity about 48.4% of that obtained with tartrate
Lentinus tigrinus
1.11.1.13
malonate
the highest stimulation of 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate, malonate also serves as a chelator for the Mn3+ chelate formation, leading to a specific activity about 54.8% of that obtained with tartrate
Lentinus tigrinus
1.11.1.13
oxalate
oxalate serves as a chelator for the Mn3+ chelate formation, leading to a specific activity about 64.7% of that obtained with tartrate
Lentinus tigrinus
1.11.1.13
Tartrate
the highest rate of Mn3+ formation is obtained with 10 mM tartrate
Lentinus tigrinus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.11.1.13
heme
the heme content is 0.96 mol per mole of the enzyme
Lentinus tigrinus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.11.1.13
0.016
-
H2O2
; pH 5.5, 45°C
Lentinus tigrinus
1.11.1.13
0.0713
-
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
Lentinus tigrinus
1.11.1.13
0.0713
-
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) Mn2+
-
Lentinus tigrinus
1.11.1.13
0.076
-
2,6-dimethoxyphenol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
0.124
-
Mn2+
; pH 5.5, 45°C
Lentinus tigrinus
1.11.1.13
0.196
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
Lentinus tigrinus
1.11.1.13
0.196
-
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
0.213
-
guaiacol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
0.293
-
pyrogallol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
0.56
-
veratryl alcohol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
2.3
-
guaiacol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
Lentinus tigrinus
1.11.1.13
3.74
-
pyrogallol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
Lentinus tigrinus
1.11.1.13
6.954
-
2,6-dimethoxyphenol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
Lentinus tigrinus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.11.1.13
Manganese
-
Lentinus tigrinus
1.11.1.13
Mn2+
veratryl alcohol oxidation requires the simultaneous presence of H2O2 and Mn2+
Lentinus tigrinus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.1.13
50500
-
determined by gel filtration and SDS-PAGE; gel filtration
Lentinus tigrinus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.11.1.13
Mn2+ + H+ + H2O2
Lentinus tigrinus
-
Mn3+ + H2O
-
-
?
1.11.1.13
Mn2+ + H+ + H2O2
Lentinus tigrinus CBS 577.79
-
Mn3+ + H2O
-
-
?
1.11.1.13
Mn2+ + H+ + H2O2
Lentinus tigrinus 577.79
-
Mn3+ + H2O
-
-
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
1.11.1.13
glycoprotein
N-glycosylation of about 5.3% of the high-mannose type; the enzyme displays an extent of N-glycosylation of about 5.3% of the high-mannose type
Lentinus tigrinus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.1.13
purification scheme involves ultrafiltration, affinity chromatography on concanavalin-A Sepharose and gel filtration; ultrafiltration, Concanavalin-A Sepharose column chromatography and Superdex 75 gel filtration
Lentinus tigrinus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.11.1.13
40
-
culture supernatant, pH 5.5, 45°C; purification step culture supernatant
Lentinus tigrinus
1.11.1.13
41
-
purification step ultrafiltration
Lentinus tigrinus
1.11.1.13
169
-
purification step chromatography on a concanavalin-A Sepharose column
Lentinus tigrinus
1.11.1.13
288
-
after 7.2fold purification, pH 5.5, 45°C; purification step gel filtration
Lentinus tigrinus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.1.13
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + Mn2+ + ?
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + Mn2+ + ?
-
699016
Lentinus tigrinus CBS 577.79
?
-
-
-
?
1.11.1.13
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + Mn2+ + ?
-
699016
Lentinus tigrinus 577.79
?
-
-
-
?
1.11.1.13
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid + H2O2
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid + H2O2 + Mn2+
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
2,6-dimethoxyphenol + H2O2
the highest relative activity for 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
2,6-dimethoxyphenol + H2O2
the highest relative activity for 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate
699016
Lentinus tigrinus CBS 577.79
?
-
-
-
?
1.11.1.13
2,6-dimethoxyphenol + H2O2
the highest relative activity for 2,6-dimethoxyphenol oxidation is observed in the presence of 10 mM malonate
699016
Lentinus tigrinus 577.79
?
-
-
-
?
1.11.1.13
2,6-dimethoxyphenol + H2O2 + Mn2+
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
2,6-dimethoxyphenol + Mn2+ + ?
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
guaiacol + H2O2
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
guaiacol + H2O2
-
699016
Lentinus tigrinus CBS 577.79
?
-
-
-
?
1.11.1.13
guaiacol + H2O2
-
699016
Lentinus tigrinus 577.79
?
-
-
-
?
1.11.1.13
guaiacol + H2O2 + Mn2+
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
guaiacol + Mn2+ + ?
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
Mn2+ + H+ + H2O2
-
699016
Lentinus tigrinus
Mn3+ + H2O
-
-
-
?
1.11.1.13
Mn2+ + H+ + H2O2
-
699016
Lentinus tigrinus CBS 577.79
Mn3+ + H2O
-
-
-
?
1.11.1.13
Mn2+ + H+ + H2O2
-
699016
Lentinus tigrinus 577.79
Mn3+ + H2O
-
-
-
?
1.11.1.13
pyrogallol + H2O2
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
pyrogallol + H2O2
-
699016
Lentinus tigrinus CBS 577.79
?
-
-
-
?
1.11.1.13
pyrogallol + H2O2
-
699016
Lentinus tigrinus 577.79
?
-
-
-
?
1.11.1.13
pyrogallol + H2O2 + Mn2+
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
pyrogallol + Mn2+ + ?
-
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
veratryl alcohol + H2O2 + Mn2+
veratryl alcohol oxidation requires the simultaneous presence of H2O2 and Mn2+
699016
Lentinus tigrinus
?
-
-
-
?
1.11.1.13
veratryl alcohol + Mn2+ + ?
-
699016
Lentinus tigrinus
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.11.1.13
monomer
; 1 * 50500, SDS-PAGE
Lentinus tigrinus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.11.1.13
45
-
; optimum temperature for the oxidation of Mn2+ and DMOP
Lentinus tigrinus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.11.1.13
10
60
the enzyme shows relative activities of 34, 50, and 47% at 10, 40, and 60°C, respectively
Lentinus tigrinus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.11.1.13
50
70
the enzyme is rather unstable at 50, 60, and 70°C, resulting in half-lives of 660, 105, and 41 s, respectively
Lentinus tigrinus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.11.1.13
0.45
-
veratryl alcohol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
0.74
-
guaiacol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
Lentinus tigrinus
1.11.1.13
1.87
-
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
Lentinus tigrinus
1.11.1.13
1.87
-
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) Mn2+
-
Lentinus tigrinus
1.11.1.13
5.67
-
2,6-dimethoxyphenol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
Lentinus tigrinus
1.11.1.13
8.92
-
guaiacol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
9.98
-
pyrogallol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
Lentinus tigrinus
1.11.1.13
20.2
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
Lentinus tigrinus
1.11.1.13
20.2
-
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
20.95
-
pyrogallol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
26.26
-
2,6-dimethoxyphenol
; in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
Lentinus tigrinus
1.11.1.13
121
-
H2O2
; with 1.5 mM Mn2+ as cosubstrate, pH 5.5, 45°C
Lentinus tigrinus
1.11.1.13
242.4
-
Mn2+
; with 0.2 mM H2O2 as cosubstrate, pH 5.5, 45°C
Lentinus tigrinus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.11.1.13
3
-
optimal pH for the Mn-dependent oxidation of veratryl alcohol; optimum pH for the Mn2+-dependent oxidation of veratryl alehyde
Lentinus tigrinus
1.11.1.13
3.5
4.5
pH optimum for Mn2+-independent DMOP oxidation
Lentinus tigrinus
1.11.1.13
4
4.5
pH optimum for Mn2+-dependent DMOP oxidation
Lentinus tigrinus
1.11.1.13
5.5
-
-
Lentinus tigrinus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.11.1.13
3.5
4.5
optima for Mn2+-independent 2,6-dimethoxyphenol oxidation
Lentinus tigrinus
1.11.1.13
3.5
6.5
Mn2+ oxidation shows an optimum in the range 5.5-5.75 and negligible activity at pH values below 3.5 and above 6.5
Lentinus tigrinus
1.11.1.13
4
4.5
optima for Mn2+-dependent 2,6-dimethoxyphenol oxidation
Lentinus tigrinus
1.11.1.13
5.5
5.8
the pH-activity profile for Mn2+ oxidation shows an optimum in the range 5.5 - 5.75 and negligible activity at pHs below 3.5 and above 6.5
Lentinus tigrinus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.11.1.13
3.5
5.5
the enzyme shows a half-life of 8.8 h at pH 3.5, the half-lives of MnP activity at pH 4.5, 5.0, and 5.5 are 89.5, 99, and 101 h, respectively
Lentinus tigrinus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.11.1.13
Lentinus tigrinus
-
-
4.07
1.11.1.13
Lentinus tigrinus
isoelectric focusing
-
4.1