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Literature summary extracted from

  • Menon, B.R.; Davison, P.A.; Hunter, C.N.; Scrutton, N.S.; Heyes, D.J.
    Mutagenesis alters the catalytic mechanism of the light-driven enzyme protochlorophyllide oxidoreductase (2009), J. Biol. Chem., 285, 2113-2119.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.33 expressed in Escherichia coli Thermosynechococcus vestitus
1.3.1.33 expression in Escherichia coli cells Thermosynechococcus vestitus

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.33 C199/C226S mutant, mutation of the absolutely conserved cysteine residues Thermosynechococcus vestitus
1.3.1.33 C199S mutant, Cys199 has a relatively minor role in catalysis Thermosynechococcus vestitus
1.3.1.33 C199S kcat comparable to wild-type, relative activity comparable to wild-type, Kd (NADPH) comparable to wild-type, Kd (protochlorophyllide) comparable to wild-type Thermosynechococcus vestitus
1.3.1.33 C199S/C226S kcat more that 10fold decreased to wild-type, relative activity highly decreased compared to wild-type, Kd (NADPH) 2fold increased compared to wild-type, Kd (protochlorophyllide) 6fold increased compared to wild-type Thermosynechococcus vestitus
1.3.1.33 C226S mutant, mutation causes a remarkable change in the mechansim of the hydrogen transfer reactions Thermosynechococcus vestitus
1.3.1.33 C226S kcat more that 10fold decreased to wild-type, relative activity highly decreased compared to wild-type, Kd (NADPH) comparable to wild-type, Kd (protochlorophyllide) 4fold increased compared to wild-type Thermosynechococcus vestitus
1.3.1.33 C37S mutant, Cys37 has a relatively minor role in catalysis Thermosynechococcus vestitus
1.3.1.33 C37S kcat comparable to wild-type, relative activity comparable to wild-type, Kd (NADPH) increased compared to wild-type, Kd (protochlorophyllide) equal to wild-type Thermosynechococcus vestitus
1.3.1.33 C37S/C199S mutant, mutation of the absolutely conserved cysteine residues Thermosynechococcus vestitus
1.3.1.33 C37S/C199S kcat comparable to wild-type, relative activity comparable to wild-type, Kd (NADPH) 10fold increased compared to wild-type, Kd (protochlorophyllide) comparable to wild-type Thermosynechococcus vestitus
1.3.1.33 C37S/C199S/C226S mutant, mutation of the absolutely conserved cysteine residues Thermosynechococcus vestitus
1.3.1.33 C37S/C199S/C226S kcat more that 10fold decreased to wild-type, relative activity highly decreased compared to wild-type, Kd (NADPH) 10fold increased compared to wild-type, Kd (protochlorophyllide) 6fold increased compared to wild-type Thermosynechococcus vestitus
1.3.1.33 C37S/C226S mutant, mutation of the absolutely conserved cysteine residues Thermosynechococcus vestitus
1.3.1.33 C37S/C226S kcat more that 10fold decreased to wild-type, relative activity highly decreased compared to wild-type, Kd (NADPH) 10fold increased compared to wild-type, Kd (protochlorophyllide) 6fold increased compared to wild-type Thermosynechococcus vestitus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.33 additional information
-
 protochlorophyllide mutant C199/C226S, relative activity 6.6% Thermosynechococcus vestitus
1.3.1.33 additional information
-
 protochlorophyllide mutant C199S, relative activity 98.2% Thermosynechococcus vestitus
1.3.1.33 additional information
-
 protochlorophyllide mutant C226S, relative activity 7.2% Thermosynechococcus vestitus
1.3.1.33 additional information
-
 protochlorophyllide mutant C37S, relative activity 99.4% Thermosynechococcus vestitus
1.3.1.33 additional information
-
 protochlorophyllide mutant C37S/C199S, relative activity 98.8% Thermosynechococcus vestitus
1.3.1.33 additional information
-
 protochlorophyllide mutant C37S/C199S/C226S, relative activity 7.1% Thermosynechococcus vestitus
1.3.1.33 additional information
-
 protochlorophyllide mutant C37S/C226S, relative activity 6.8% Thermosynechococcus vestitus
1.3.1.33 additional information
-
 protochlorophyllide wild type enzyme, relative activity 100% Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH Thermosynechococcus vestitus
-
 chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH + H+ Thermosynechococcus vestitus activation by light chlorophyllide + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.33 Thermosynechococcus vestitus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH
-
 Thermosynechococcus vestitus chlorophyllide + NADP+
-
 ?
1.3.1.33 protochlorophyllide + NADPH + H+ activation by light Thermosynechococcus vestitus chlorophyllide + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.33 POR
-
 Thermosynechococcus vestitus
1.3.1.33 protochlorophyllide oxidoreductase
-
 Thermosynechococcus vestitus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.33 25
-
 assay at Thermosynechococcus vestitus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.33 0.011
-
 protochlorophyllide mutant C199/C226S Thermosynechococcus vestitus
1.3.1.33 0.011
-
 protochlorophyllide pH 7.5, 25°C, mutant C199S/C226S, co-substrate: NADPH Thermosynechococcus vestitus
1.3.1.33 0.011
-
 NADPH pH 7.5, 25°C, mutant C199S/C226S, co-substrate: protochlorophyllide Thermosynechococcus vestitus
1.3.1.33 0.0113
-
 protochlorophyllide mutant C37S/C226S Thermosynechococcus vestitus
1.3.1.33 0.0113
-
 protochlorophyllide pH 7.5, 25°C, mutant C37S/C226S, co-substrate: NADPH Thermosynechococcus vestitus
1.3.1.33 0.0113
-
 NADPH pH 7.5, 25°C, mutant C37S/C226S, co-substrate: protochlorophyllide Thermosynechococcus vestitus
1.3.1.33 0.0117
-
 protochlorophyllide mutant C37S/C199S/C226S Thermosynechococcus vestitus
1.3.1.33 0.0117
-
 protochlorophyllide pH 7.5, 25°C, mutant C37S/C199S/C226S, co-substrate: NADPH Thermosynechococcus vestitus
1.3.1.33 0.0117
-
 NADPH pH 7.5, 25°C, mutant C37S/C199S/C226S, co-substrate: protochlorophyllide Thermosynechococcus vestitus
1.3.1.33 0.0118
-
 protochlorophyllide mutant C226S Thermosynechococcus vestitus
1.3.1.33 0.0118
-
 protochlorophyllide pH 7.5, 25°C, mutant C226S, co-substrate: NADPH Thermosynechococcus vestitus
1.3.1.33 0.0118
-
 NADPH pH 7.5, 25°C, mutant C226S, co-substrate: protochlorophyllide Thermosynechococcus vestitus
1.3.1.33 0.162
-
 protochlorophyllide mutant C199S Thermosynechococcus vestitus
1.3.1.33 0.162
-
 protochlorophyllide pH 7.5, 25°C, mutant C199S, co-substrate: NADPH Thermosynechococcus vestitus
1.3.1.33 0.162
-
 NADPH pH 7.5, 25°C, mutant C199S, co-substrate: protochlorophyllide Thermosynechococcus vestitus
1.3.1.33 0.163
-
 protochlorophyllide mutant C37S/C199S Thermosynechococcus vestitus
1.3.1.33 0.164
-
 protochlorophyllide mutant C37S Thermosynechococcus vestitus
1.3.1.33 0.164
-
 protochlorophyllide pH 7.5, 25°C, mutant C37S, co-substrate: NADPH Thermosynechococcus vestitus
1.3.1.33 0.164
-
 NADPH pH 7.5, 25°C, mutant C37S, co-substrate: protochlorophyllide Thermosynechococcus vestitus
1.3.1.33 0.165
-
 protochlorophyllide wild type enzyme Thermosynechococcus vestitus
1.3.1.33 0.165
-
 protochlorophyllide pH 7.5, 25°C, wild-type, co-substrate: NADPH Thermosynechococcus vestitus
1.3.1.33 0.165
-
 NADPH pH 7.5, 25°C, wild-type, co-substrate: protochlorophyllide Thermosynechococcus vestitus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.33 7.5
-
 assay at Thermosynechococcus vestitus
1.3.1.33 7.5
-
 activity assay Thermosynechococcus vestitus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.33 NADPH
-
 Thermosynechococcus vestitus