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Literature summary extracted from
- Engineering and characterization of the ribosomal L10.L12 stalk complex. A structural element responsible for high turnover of the elongation factor G-dependent GTPase (2009), J. Biol. Chem., 284, 85-92.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.6.5.3 | expression in Escherichia coli BL21 | Thermus thermophilus |
| 3.6.5.3 | expression in Escherichia coli BL21 | Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.6.5.3 | additional information | used as a hybrid sytem with Escherichia coli ribsomal proteins L10, L11, L12 | Thermus thermophilus |
| 3.6.5.3 | additional information | used as a hybrid sytem with Thermus thermophilus ribsomal protein L11 | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.5.3 | Escherichia coli | - |
- |
- |
| 3.6.5.3 | Escherichia coli | - |
used in hybrid system with Thermus thermophilus ribosomal protein L11 - EC 3.6.5.3 | - |
| 3.6.5.3 | Thermus thermophilus | - |
used in hybrid system with Escherichia coli ribosomal proteins L10, L11, L12 - EC 3.6.5.3 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.5.3 | DEAE Sephadex A-50 column | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.5.3 | GTP + H2O | 0.5 mM GTP, 37°C, 10 min | Thermus thermophilus | GDP + phosphate | - |
? |  |
| 3.6.5.3 | GTP + H2O | 0.5 mM GTP, 37°C, 10 min | Escherichia coli | GDP + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.6.5.3 | heptamer | - |
Thermus thermophilus |
| 3.6.5.3 | pentamer | - |
Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.6.5.3 | Ec-L10 | - |
Escherichia coli |
| 3.6.5.3 | Ec-L11 | - |
Escherichia coli |
| 3.6.5.3 | Ec-L12 | - |
Escherichia coli |
| 3.6.5.3 | EF-G | - |
Escherichia coli |
| 3.6.5.3 | elongation factor | - |
Escherichia coli |
| 3.6.5.3 | L10 | - |
Escherichia coli |
| 3.6.5.3 | L11 | - |
Thermus thermophilus |
| 3.6.5.3 | L11 | - |
Escherichia coli |
| 3.6.5.3 | L12 | - |
Escherichia coli |
| 3.6.5.3 | Tt-L11 | - |
Thermus thermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.5.3 | 37 | - |
assay at | Thermus thermophilus |
| 3.6.5.3 | 37 | - |
assay at | Escherichia coli |
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Release 2023.1 (January 2023)
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