EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.3.15 | drug development | in humans the enzyme is a potential drug target for treatment of primary hyperoxaluria, a genetic disorder where overproduction of oxalate results in the formation of kidney stones | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.3.15 | H260A | site-directed mutagenesis | Homo sapiens |
1.1.3.15 | H260Q | site-directed mutagenesis | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.15 | chloride | inhibits the enzyme at high concentrations | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.15 | additional information | - |
additional information | stopped-flow, steady-state, and presteady-state kinetics at different pH values, pH profiles, detailed overview | Homo sapiens | |
1.1.3.15 | 0.2 | - |
glycolate | pH 7.5, 30°C, recombinant enzyme, first order | Homo sapiens | |
1.1.3.15 | 0.23 | - |
glycolate | pH 7.0, 30°C, recombinant enzyme, first order | Homo sapiens | |
1.1.3.15 | 0.44 | - |
O2 | pH 7.5, 30°C, recombinant enzyme, first order | Homo sapiens | |
1.1.3.15 | 0.59 | - |
O2 | pH 9.0, 30°C, recombinant enzyme, first order | Homo sapiens | |
1.1.3.15 | 0.64 | - |
O2 | pH 7.0, 30°C, recombinant enzyme, first order | Homo sapiens | |
1.1.3.15 | 2 | - |
glycolate | pH 9.0, 30°C, recombinant enzyme, first order | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.3.15 | peroxisome | - |
Homo sapiens | 5777 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.15 | glycolate + O2 | Homo sapiens | - |
glyoxylate + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.15 | Homo sapiens | Q9UJM8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.3.15 | recombinant enzyme by nickel affinity chromatography, ammonium sulfate fractionation and dialysis | Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.3.15 | an (S)-2-hydroxy carboxylate + O2 = a 2-oxo carboxylate + H2O2 | the pH affects the kinetic steps of the catalytic mechanism of human glycolate oxidase, the enzyme shows a ping-pong bi-bi kinetic mechanism between pH 6.0 and 10.0, overview. Formation of the enzyme-substrate complex suggests the presence of a protonated group participating in substrate binding | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.3.15 | liver | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.15 | glycolate + O2 | - |
Homo sapiens | glyoxylate + H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.3.15 | glycolate oxidase | - |
Homo sapiens |
1.1.3.15 | GOX | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.15 | 30 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.15 | additional information | - |
additional information | a second-order rate constant for the capture of glycolate (kcat/Kglycolate) of 68300 M/s, and a second-order rate constant for reaction of the reduced flavin with oxygen (kcat/Koxygen) of 24500 Ms | Homo sapiens | |
1.1.3.15 | 15.7 | - |
glycolate | pH 7.0, 30°C, recombinant enzyme, first order | Homo sapiens | |
1.1.3.15 | 20 | - |
glycolate | pH 7.5, 30°C, recombinant enzyme, first order | Homo sapiens | |
1.1.3.15 | 31.2 | - |
glycolate | pH 9.0, 30°C, recombinant enzyme, first order | Homo sapiens | |
1.1.3.15 | 54.1 | - |
flavin | pH 7.0, 30°C, flavin reduction | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.15 | 7.5 | - |
- |
Homo sapiens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.15 | 5 | 10 | pH profiles, the enzyme shows a ping-pong bi-bi kinetic mechanism between pH 6.0 and 10.0, overview | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.15 | flavin | dependent on | Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.15 | 280 | - |
chloride | pH 7.0, 25°C | Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.3.15 | malfunction | the enzyme is involved in primary hyperoxaluria, a genetic disorder where overproduction of oxalate results in the formation of kidney stones | Homo sapiens |